Caulfield M J, Shaffer D
J Immunol. 1987 Jun 1;138(11):3680-3.
Specific immune complexes, prepared at different ratios of antibody to antigen, were examined for their effects on the antibody response of BALB/c mice to the cell wall polysaccharide antigen (PnC) extracted from Streptococcus pneumonia R36a. Mice immunized with complexes formed in antigen excess developed a PnC-specific antibody response that was equivalent to that in mice injected with free antigen. On the other hand, mice injected with complexes formed in antibody excess developed very little PnC-specific antibody. Furthermore, administration of immune complexes (formed in antibody excess) resulted in suppression of the response to an immunogenic dose of PnC given concurrently or 1 day after injection of immune complexes but not when the antigen was given 1 day before injection of the immune complexes. Injections of free antibody (TEPC-15) also resulted in suppression of the response to antigenic challenge; however, suppression was greatest when the antibody was injected concurrently with the antigen, suggesting that the suppression was mediated through the formation of immune complexes in vivo. The suppression appears to be specific for the antigen (PnC), since in mice injected with TEPC-15/PnC complexes (formed in antibody excess) and challenged with PnC coupled to sheep RBC, only the response to PnC was suppressed.
制备了不同抗体与抗原比例的特异性免疫复合物，检测其对BALB/c小鼠针对从肺炎链球菌R36a提取的细胞壁多糖抗原（PnC）的抗体反应的影响。用抗原过量形成的复合物免疫的小鼠产生了与注射游离抗原的小鼠相当的PnC特异性抗体反应。另一方面，注射抗体过量形成的复合物的小鼠产生的PnC特异性抗体很少。此外，给予（抗体过量形成的）免疫复合物会抑制对同时给予或在注射免疫复合物后1天给予的免疫原性剂量的PnC的反应，但在抗原在注射免疫复合物前1天给予时则不会。注射游离抗体（TEPC-15）也会导致对抗原攻击的反应受到抑制；然而，当抗体与抗原同时注射时抑制作用最大，这表明抑制是通过体内免疫复合物的形成介导的。这种抑制似乎对抗原（PnC）具有特异性，因为在注射了TEPC-15/PnC复合物（抗体过量形成）并用与绵羊红细胞偶联的PnC攻击的小鼠中，只有对PnC的反应受到抑制。
