Van Yüzüncü Yıl Üniversitesi Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, 65080 Zeve, Van, Türkiye.
Jt Dis Relat Surg. 2022;33(2):374-384. doi: 10.52312/jdrs.2022.640. Epub 2022 Jul 6.
The aim of this study was to investigate the radiological, biomechanical, histopathological and immunohistochemical effects of theranekron on fracture healing in an experimental rat model.
Forty-eight male albino Wistar rats were used. Four groups were formed, with 12 rats in each of theranekron groups 1 and 2, and control groups 1 and 2. After a fracture was created in the right femur of the rats included in the study, fixation was performed with an intramedullary Kirschner wire. Theranekron was administered subcutaneously to theranekron groups 1 and 2 at a dose of 0.3 mg/kg on days 0, 5 and 10. After radiographic analysis of the femurs of theranekron group 1 and control group 1 rats at four weeks of the study was performed, both groups were divided into two equal subgroups (six femurs in each group). Histopathological and immunohistochemical examinations were performed in one subgroup and biomechanical examination in the other subgroup. At the end of six weeks, the rats in theranekron group 2 and control group 2 were evaluated after applying the same procedure as in the fourth week.
When the mean radiological scores of the theranekron and control groups were compared, a statistically significant difference was found in favor of the theranekron group at four and six weeks (p=0.028 and p=0.006, respectively). At four weeks, statistically significant higher biomechanical forces were obtained in the theranekron group compared to the control group (p=0.030). In the histopathological evaluation, the inflammation value of the control group at four weeks was statistically significantly higher than the theranekron group (p=0.027). The angiogenesis, osteoblast proliferation, and bone formation values of the theranekron group were significantly higher than the control group (p=0.014, p=0.014, and p=0.005, respectively). At six weeks, the bone formation values of the theranekron group were statistically significantly higher than the control group (p=0.021). The difference between the theranekron group and the control group scores of the immunohistochemical evaluation were statistically significantly different at four and six weeks (p=0.006 and p=0.011, respectively).
Theranekron may play a role in accelerating fracture healing by reducing acute inflammation process in the early period of fracture union, increasing fracture strength, angiogenesis, osteoblast proliferation, and bone formation.
本研究旨在通过实验性大鼠模型探讨 theranekron 对骨折愈合的放射学、生物力学、组织病理学和免疫组织化学影响。
共使用 48 只雄性白化 Wistar 大鼠。将它们分为 4 组，theranekron 组 1 和 2 以及对照组 1 和 2 各有 12 只大鼠。在研究纳入的大鼠右侧股骨造成骨折后，使用髓内克氏针进行固定。在研究的第 4 周，对 theranekron 组 1 和对照组 1 大鼠的股骨进行放射学分析后，将两组均分为两个相等的亚组（每组 6 个股骨）。对其中一个亚组进行组织病理学和免疫组织化学检查，对另一个亚组进行生物力学检查。在研究的第 6 周末，对 theranekron 组 2 和对照组 2 大鼠进行相同的处理后进行评估。
当比较 theranekron 组和对照组的平均放射学评分时，发现 theranekron 组在第 4 周和第 6 周有统计学意义上的优势（p=0.028 和 p=0.006）。在第 4 周，与对照组相比，theranekron 组获得的生物力学力明显更高（p=0.030）。在组织病理学评估中，第 4 周对照组的炎症值明显高于 theranekron 组（p=0.027）。theranekron 组的血管生成、成骨细胞增殖和骨形成值明显高于对照组（p=0.014、p=0.014 和 p=0.005）。在第 6 周，theranekron 组的骨形成值明显高于对照组（p=0.021）。在第 4 周和第 6 周，theranekron 组和对照组的免疫组织化学评估评分之间的差异具有统计学意义（p=0.006 和 p=0.011）。
Theranekron 通过减少骨折愈合早期的急性炎症过程、增加骨折强度、血管生成、成骨细胞增殖和骨形成，可能在加速骨折愈合中发挥作用。
