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灌木醇 A 通过靶向成骨细胞分化中的 BMP2-Smad/1/5/8-RUNX2 促进前成骨细胞的分化。

Suffruticosol A elevates osteoblast differentiation targeting BMP2-Smad/1/5/8-RUNX2 in pre-osteoblasts.

机构信息

Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.

National Development Institute of Korean Medicine, Gyeongsan, Republic of Korea.

出版信息

Biofactors. 2023 Jan;49(1):127-139. doi: 10.1002/biof.1878. Epub 2022 Jul 19.

DOI:10.1002/biof.1878
PMID:35852295
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10947220/
Abstract

The Paeonia suffruticosa ANDR. (P. suffruticosa) is commonly used in traditional medicine for various purposes. Suffruticosol A (Suf-A), isolated from P. suffruticosa, is a beneficial compound with antibiofilm, antivirulence, and anti-inflammatory properties. The aim of the present study was to investigate the biological effects of Suf-A on osteogenic processes in pre-osteoblasts. It was determined here in that Suf-A (>98.02%), isolated from P. suffruticosa, showed no cytotoxicity at 0.1-30 μM; however, it induced cytotoxicity at 50-100 μM in pre-osteoblasts. Suf-A increased osteogenic alkaline phosphatase activity and expression levels of noncollagenous proteins. Adhesion and trans-migration on the extracellular matrix were potentiated by Suf-A, but not by wound-healing migration. Suf-A did not affect autophagy or necroptosis during osteoblast differentiation. We found that Suf-A increased runt-related transcription factor 2 (RUNX2) levels and mineralized matrix formation. RUNX2 expression was mediated by Suf-A-induced BMP2-Smad1/5/8 and mitogen-activated protein kinase signaling, as demonstrated by Noggin, a BMP2 inhibitor. These results suggest that Suf-A is a potential natural osteogenic compound.

摘要

牡丹皮（Paeonia suffruticosa ANDR.）在传统医学中常用于多种用途。从牡丹皮中分离得到的牡丹酚 A（Suf-A）是一种有益的化合物，具有抗生物膜、抗病毒和抗炎特性。本研究旨在探讨 Suf-A 对成骨前体细胞成骨过程的生物学影响。结果表明，Suf-A（>98.02%）从牡丹皮中分离出来，在 0.1-30μM 时没有细胞毒性，但在 50-100μM 时对成骨前体细胞表现出细胞毒性。Suf-A 增加了成碱性磷酸酶活性和非胶原蛋白的表达水平。Suf-A 增强了细胞在细胞外基质上的黏附和迁移，但对创伤愈合迁移没有影响。Suf-A 在成骨细胞分化过程中不影响自噬或坏死。我们发现 Suf-A 增加了 runt 相关转录因子 2（RUNX2）的水平和矿化基质的形成。BMP2 抑制剂 Noggin 表明，Suf-A 通过诱导 BMP2-Smad1/5/8 和丝裂原活化蛋白激酶信号通路来介导 RUNX2 的表达。这些结果表明 Suf-A 是一种有潜力的天然成骨化合物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6b/10947220/bba14e242d11/BIOF-49-127-g005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6b/10947220/bba14e242d11/BIOF-49-127-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6b/10947220/ed31319caf89/BIOF-49-127-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6b/10947220/2a90ecfb0f60/BIOF-49-127-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6b/10947220/672a2508087c/BIOF-49-127-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3a6b/10947220/bba14e242d11/BIOF-49-127-g005.jpg

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