Department of Agrofood Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun 55365, Republic of Korea.
Department of Food and Biotechnology, Korea University, Sejong City 30019, Republic of Korea.
Nutrients. 2023 Oct 16;15(20):4372. doi: 10.3390/nu15204372.
Sword bean (SB) contains various phytochemicals, such as flavonoids, tannins, saponins, and terpenoids. Although the evaluation of its potential functions, including antioxidant, anti-obesity, anti-inflammatory, liver protection, and antiangiogenic activities, has been widely reported, research on their use in osteoporosis prevention is insufficient. Furthermore, while various studies are conducted on SB, research on sword bean pods (SBP) is not yet active, and little is known about it. Therefore, this study investigated the effects of promoting osteoblast differentiation of MC3T3-E1 cells using SB and SBP extracts and their mechanisms. We show that SBP extracts increase osteoblast proliferation, mineralization-activated alkaline phosphatase (ALP), and collagen synthesis activities. Additionally, treatment with SBP extract increased the expression of markers related to osteoblast differentiation, such as ALP, SPARC, RUNX2, COL-I, BMP2, OCN, and OPN. It was confirmed that SBP induces differentiation by activating the BMP2/SMAD/RUNX2 pathway. We also show that SBP is more effective than SB, and SBP may be useful in assimilating bone minerals and preventing osteoporosis.
刀豆含有多种植物化学物质，如类黄酮、单宁、皂苷和萜类化合物。虽然已经广泛报道了其潜在功能的评估，包括抗氧化、抗肥胖、抗炎、保肝和抗血管生成活性，但在骨质疏松症预防方面的研究还不够。此外，虽然对 SB 进行了各种研究，但对刀豆荚（SBP）的研究并不活跃，对其了解甚少。因此，本研究探讨了 SB 和 SBP 提取物促进 MC3T3-E1 细胞成骨分化的作用及其机制。我们发现 SBP 提取物可增加成骨细胞增殖、矿化激活碱性磷酸酶（ALP）和胶原合成活性。此外，SBP 提取物处理可增加与成骨细胞分化相关的标志物的表达，如 ALP、SPARC、RUNX2、COL-I、BMP2、OCN 和 OPN。证实 SBP 通过激活 BMP2/SMAD/RUNX2 途径诱导分化。我们还表明，SBP 比 SB 更有效，SBP 可能有助于同化骨矿物质和预防骨质疏松症。
