Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul 02447, Korea.
National Institute for Korean Medicine Development, Gyeongsan 38540, Korea.
Int J Mol Sci. 2021 Jun 27;22(13):6899. doi: 10.3390/ijms22136899.
is a magnificent and long-lived woody plant that has traditionally been used to treat various diseases including inflammatory, neurological, cancer, and cardiovascular diseases. In the present study, we demonstrated the biological mechanisms of paeonoside (PASI) isolated from the dried roots of in pre-osteoblasts. Herein, we found that PASI has no cytotoxic effects on pre-osteoblasts. Migration assay showed that PASI promoted wound healing and transmigration in osteoblast differentiation. PASI increased early osteoblast differentiation and mineralized nodule formation. In addition, PASI enhanced the expression of Wnt3a and bone morphogenetic protein 2 (BMP2) and activated their downstream molecules, Smad1/5/8 and β-catenin, leading to increases in runt-related transcription factor 2 (RUNX2) expression during osteoblast differentiation. Furthermore, PASI-mediated osteoblast differentiation was attenuated by inhibiting the BMP2 and Wnt3a pathways, which was accompanied by reduction in the expression of RUNX2 in the nucleus. Taken together, our findings provide evidence that PASI enhances osteoblast differentiation and mineralized nodules by regulating RUNX2 expression through the BMP2 and Wnt3a pathways, suggesting a potential role for PASI targeting osteoblasts to treat bone diseases including osteoporosis and periodontitis.
是一种壮丽而长寿的木本植物，传统上被用于治疗各种疾病，包括炎症、神经、癌症和心血管疾病。在本研究中，我们在成骨前体细胞中证明了来自 的干根分离的牡丹皮苷（PASI）的生物学机制。在此，我们发现 PASI 对成骨前体细胞没有细胞毒性作用。迁移实验表明，PASI 促进了成骨细胞分化中的伤口愈合和迁移。PASI 增加了早期成骨细胞分化和矿化结节形成。此外，PASI 增强了 Wnt3a 和骨形态发生蛋白 2（BMP2）的表达，并激活了它们的下游分子 Smad1/5/8 和 β-连环蛋白，导致成骨细胞分化过程中 runt 相关转录因子 2（RUNX2）的表达增加。此外，通过抑制 BMP2 和 Wnt3a 途径，PASI 介导的成骨细胞分化被减弱，伴随着细胞核中 RUNX2 表达的减少。总之，我们的研究结果提供了证据，表明 PASI 通过调节 BMP2 和 Wnt3a 途径来增强成骨细胞分化和矿化结节，表明 PASI 靶向成骨细胞治疗骨质疏松症和牙周炎等骨骼疾病的潜力。
