State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, PR China.
College of Marine Life Sciences, and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, 266003, PR China.
Int J Syst Evol Microbiol. 2022 Jul;72(7). doi: 10.1099/ijsem.0.005455.
A Gram-stain-negative, aerobic, flagellated and rod-shaped bacterium, designated strain SM2107, was isolated from a deep-sea sediment sample collected from the Southwest Indian Ocean. Strain SM2107 grew at 4-40 °C and with 0-10.0 % (w/v) NaCl. It reduced nitrate to nitrite and hydrolysed casein, gelatin, chitin and DNA. The phylogenetic trees based on the 16S rRNA genes and single-copy orthologous clusters showed that strain SM2107, together with , and , formed a separate clade, having the highest similarity to the type strain of (98.3%). The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol and the major cellular fatty acids were summed feature 8 (C 7 and/or C 6), C, C 8 and summed feature 3 (C 7 and/or C 6). The only respiratory quinone was Q-8. The genomic DNA G+C content of strain SM2107 was 48.8 %. The digital DNA-DNA hybridization values between strain SM2107 and type strains of , and were 41.16, 37.70 and 31.80 %, while the average amino acid identity values between them were 87.59, 86.76 and 83.64 %, respectively. Based on the polyphasic evidence presented in this study, strain SM2107 was considered to represent a novel species within the genus , for which the name was proposed. The type strain is SM2107 (=MCCC M24986=KCTC 82921). Moreover, the transfer of and to the genus as comb. nov. (type strain TS-T4=CGMCC 1.12461=JCM 19264) and comb. nov. (type strain BA131=LMG 23581=CIP 109200) is also proposed.
一株革兰氏阴性、需氧、鞭毛和杆状的细菌，被命名为 SM2107 菌株，从西南印度洋深海沉积物样本中分离得到。SM2107 菌株在 4-40°C 和 0-10.0%（w/v）NaCl 的条件下生长。它可以将硝酸盐还原为亚硝酸盐，并水解酪蛋白、明胶、几丁质和 DNA。基于 16S rRNA 基因和单拷贝直系同源簇的系统发育树表明，SM2107 菌株与 、和一起形成一个单独的分支，与 （98.3%）的模式菌株最为相似。主要极性脂为磷脂酰乙醇胺和磷脂酰甘油，主要细胞脂肪酸为 C 7 和/或 C 6 组成的特征脂肪酸 8、C 16:0 和 C 8 以及 C 7 和/或 C 6 组成的特征脂肪酸 3。唯一的呼吸醌为 Q-8。SM2107 菌株的基因组 DNA G+C 含量为 48.8%。SM2107 菌株与 、和的模式菌株之间的数字 DNA-DNA 杂交值分别为 41.16%、37.70%和 31.80%，而它们之间的平均氨基酸身份值分别为 87.59%、86.76%和 83.64%。基于本研究提供的多相证据，SM2107 菌株被认为代表了属内的一个新种，因此建议将其命名为 。其模式菌株为 SM2107（=MCCC M24986=KCTC 82921）。此外，还建议将 和 转移到属中，作为新组合 （模式菌株 TS-T4=CGMCC 1.12461=JCM 19264）和 （模式菌株 BA131=LMG 23581=CIP 109200）。
