College of Marine Life Sciences and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao, PR China.
Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao, PR China.
Int J Syst Evol Microbiol. 2022 Jan;72(1). doi: 10.1099/ijsem.0.005210.
Two novel Gram-stain-negative, facultative anaerobic, non-flagellated, rod-shaped bacterial strains, designated MT13 and MT32, were isolated from sediment samples collected from the Mariana Trench at a depth of 8300 m. The two strains grew at -2-30 °C (optimum, 25 °C), at pH 5.5-10.0 (optimum, pH 7.5-8.0) and with 0-15 % (w/v) NaCl (optimum, 3-6 %). They did not reduce nitrate to nitrite nor hydrolyse Tweens 40 and 80, aesculin, casein, starch and DNA. The genomic G+C contents of draft genomes of strain MT13 and MT32 were 52.2 and 54.1 m ol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains MT13 and MT32 were affiliated with the genus , with the highest similarity to the type strain of . The values of average nucleotide identity and DNA-DNA hybridization between strain MT13 and MT32, and between strain MT13 and five closely related type strains of species indicated that strains MT13 and MT32 belonged to the same species, but represented a novel species in the genus of . The major cellular fatty acids of strains MT13 and MT32 were C, summed feature 3(C 7/6) and summed feature 8 (C 7/6). Major polar lipids of strains MT13 and MT32 included phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Ubiquinone-9 was the predominant respiratory quinone. Based on data from the present polyphasic study, strains MT13 and MT32 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MT13 (=MCCC 1K06389=KCTC 82923).
从马里亚纳海沟 8300 米深处的沉积物样本中分离到两株新型革兰氏阴性、兼性厌氧、无鞭毛、杆状细菌，分别命名为 MT13 和 MT32。这两株菌在-2-30°C（最适温度，25°C）、pH5.5-10.0（最适 pH7.5-8.0）和 0-15%（w/v）NaCl（最适 3-6%NaCl）下生长。它们不能将硝酸盐还原为亚硝酸盐，也不能水解吐温 40 和 80、马栗树皮苷、酪蛋白、淀粉和 DNA。菌株 MT13 和 MT32 的基因组 G+C 含量分别为 52.2 和 54.1 mol%。基于 16S rRNA 基因序列的系统发育分析表明，菌株 MT13 和 MT32 与属的亲缘关系最近，与属的模式菌株相似度最高。菌株 MT13 和 MT32 之间、菌株 MT13 与 5 株亲缘关系密切的属内模式菌株之间的平均核苷酸同一性和 DNA-DNA 杂交值表明，菌株 MT13 和 MT32 属于同一物种，但代表了属内的一个新种。菌株 MT13 和 MT32 的主要细胞脂肪酸为 C、特征脂肪酸 3（C 7/6）和特征脂肪酸 8（C 7/6）。菌株 MT13 和 MT32 的主要极性脂包括磷脂酰甘油、磷脂酰乙醇胺和双磷脂酰甘油。泛醌-9 是主要的呼吸醌。基于多相研究的现有数据，菌株 MT13 和 MT32 代表了属的一个新种，提议将其命名为 sp. nov.。模式菌株为 MT13（=MCCC 1K06389=KCTC 82923）。
