Key Laboratory of Marine Ecosystem Dynamics, Ministry of Natural Resources & Second Institute of Oceanography, Ministry of Natural Resources, Hangzhou 310012, PR China.
School of Oceanography, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
Int J Syst Evol Microbiol. 2023 Oct;73(10). doi: 10.1099/ijsem.0.006054.
Two Gram-stain-negative, aerobic, non-motile and short-rod-shaped bacteria, designated as strains GL-53 and GL-15-2-5, were isolated from the seamount area of the West Pacific Ocean and identified using a polyphasic taxonomic approach. The growth of strains GL-53ᵀ and GL-15-2-5 occurred at pH 5.5-10.0, 4-40 °C (optimum at 28 °C) and 0-10.0 % NaCl concentrations (optimum at 0-5.0 %). On the basis of 16S rRNA gene sequence analysis, strains GL-53ᵀ and GL-15-2-5 exhibited the highest similarity to YQF-2 (98.4 %), followed by KMM 1406 (98.1 %), E407-8 (97.4 %), H29 (97.4 %), E48 (97.2 %) and SW-353 (97.2 %). Phylogenetic analysis revealed that the isolates were affiliated with the genus and represented an independent lineage. The major fatty acids were summed feature 3 (C 7 and/or C 6), C and summed feature 8 (C 7 and/or C 6). The sole isoprenoid quinone was ubiquinone 8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminophospholipid (and one unidentified glycolipid. The DNA G+C content was 48.5 mol%. The average nucleotide identity, average amino acid identity and DNA-DNA hybridization values among the genomes of strain GL-53ᵀ and the related strains in the genus were 75.5-90.1 %, 67.5-93.9 % and 21.4-41.4 %, respectively. Based on their phenotypic, chemotaxonomic and genotypic properties, the two strains were identified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GL-53 (=KCTC 82651=MCCC M20598).
两株革兰氏阴性、需氧、不运动且短杆状的细菌，菌株 GL-53 和 GL-15-2-5，从西太平洋海山区分离得到，并通过多相分类法进行鉴定。菌株 GL-53ᵀ 和 GL-15-2-5 的生长条件为 pH5.5-10.0、4-40°C（最适 28°C）和 0-10.0%NaCl 浓度（最适 0-5.0%）。基于 16S rRNA 基因序列分析，菌株 GL-53ᵀ 和 GL-15-2-5 与 YQF-2（98.4%）最为相似，其次是 KMM 1406（98.1%）、E407-8（97.4%）、H29（97.4%）、E48（97.2%）和 SW-353（97.2%）。系统发育分析表明，这些分离株与属有关，代表一个独立的谱系。主要脂肪酸为 C 7 和/或 C 6 总和特征 3、C 和总和特征 8（C 7 和/或 C 6）。唯一的异戊烯醌是泛醌 8。主要极性脂为磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基磷脂（和一种未鉴定的糖脂）。DNA G+C 含量为 48.5mol%。菌株 GL-53ᵀ 和属内相关菌株之间的平均核苷酸同一性、平均氨基酸同一性和 DNA-DNA 杂交值分别为 75.5-90.1%、67.5-93.9%和 21.4-41.4%。基于表型、化学分类和基因型特征，这两株菌被鉴定为代表属的一个新种，建议命名为 。模式菌株为 GL-53（=KCTC 82651=MCCC M20598）。
