Feng Guangxin, Han Kaining, Yang Qian, Feng Weiting, Guo Jian, Wang Jinmei, Yang Xiaoquan
Laboratory of Food Proteins and Colloids, School of Food Science and Engineering, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China.
J Agric Food Chem. 2022 Aug 3;70(30):9536-9546. doi: 10.1021/acs.jafc.2c03564. Epub 2022 Jul 19.
High consumption of polyphenol-rich green tea, coffee, fruits, and vegetables is associated with a low risk of human chronic diseases. Recent studies highlight the relevance of polyphenol-mediated gut microbiota modulation and its impact on mucus barrier. Herein, we study the direct interaction of epicatechin (EC), epigallocatechin gallate (EGCG), and tannic acid (TA) with intestinal mucin by isothermal titration calorimetry and multiple particle tracking and the impact on mucus barrier using ex vivo mucus and Caco-2/HT29-MTX cocultures. Results show that pyrogallol-containing polyphenols EGCG and TA exhibit strong binding to intestinal mucin and reinforce mucus barrier, whereas EC does not. ECGG and TA also mitigate gliadin-mediated cytotoxicity and inflammation. The chemical binding of EGCG and TA to the nucleophilic thiol groups of mucins shows their roles as cross-linkers of mucin networks. These results bring a novel understanding of the health benefits of polyphenols and provide support for the consumption of pyrogallol-containing beverages like green tea as a potential dietary therapy for gluten-related disorders.
大量摄入富含多酚的绿茶、咖啡、水果和蔬菜与人类患慢性病的低风险相关。最近的研究强调了多酚介导的肠道微生物群调节的相关性及其对黏液屏障的影响。在此，我们通过等温滴定量热法和多粒子追踪研究表儿茶素（EC）、表没食子儿茶素没食子酸酯（EGCG）和单宁酸（TA）与肠道黏蛋白的直接相互作用，以及使用离体黏液和Caco-2/HT29-MTX共培养物对黏液屏障的影响。结果表明，含邻苯三酚的多酚EGCG和TA与肠道黏蛋白表现出强烈结合并增强黏液屏障，而EC则不然。EGCG和TA还减轻了麦醇溶蛋白介导的细胞毒性和炎症。EGCG和TA与黏蛋白的亲核硫醇基团的化学结合表明它们作为黏蛋白网络交联剂的作用。这些结果为多酚的健康益处带来了新的认识，并为饮用含邻苯三酚的饮料（如绿茶）作为麸质相关疾病的潜在饮食疗法提供了支持。
