Microbial Ecology Laboratory, Biological Oceanography Division, CSIR-National Institute of Oceanography, Raj Bhavan Road, Dona Paula, Goa, 403004, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India.
Curr Microbiol. 2022 Jul 19;79(9):258. doi: 10.1007/s00284-022-02929-y.
Polyhydroxyalkanoates (PHA) being biological polymers have attracted great attention. PHA have similar properties to that of synthetic plastic and are biodegradable. To discourage plastic pollution in the environment alternative solutions to the plastic pollution has to be readily available. High cost in production of PHA limits the production of these polymers at industrial scale. Bacteria are screened for PHA from diverse niches to meet the current requirements of cheap PHA production at industrial level. The microbial biofilm formed on the surface of microplastic could be a potential source in providing bacteria of economic importance. This paper is an attempt to search microplastic niche for potential PHA producers. PHA production variation was observed with different parameters such as type of carbon source, nitrogen source concentration and also time of incubation. Bacillus sp. CM27 showed maximum PHA yield up to 32.1% among other isolates at 48 h with 2% glucose as carbon source. Optimization of media leads to increase in PHA yield (37.69%) of CDW in Bacillus sp. CM27. Amino acid sequence of Bacillus sp.CM27 showed the presence of PhaC box with sequence, G-Y-C-M-G-G having cysteine in the middle of the box. The extracted polymer was confirmed by FTIR spectroscopy.
聚羟基烷酸酯（PHA）作为生物聚合物引起了极大的关注。PHA 具有与合成塑料相似的性质，并且可生物降解。为了遏制环境中的塑料污染，必须随时提供替代塑料污染的解决方案。PHA 生产成本高，限制了这些聚合物在工业规模上的生产。从各种生境中筛选出 PHA 的细菌，以满足在工业水平上生产廉价 PHA 的当前要求。微生物生物膜在微塑料表面形成，这可能是提供具有经济重要性的细菌的潜在来源。本文试图在微塑料生境中寻找潜在的 PHA 生产者。观察到不同参数（如碳源类型、氮源浓度以及培养时间）对 PHA 生产的影响。在 48 小时内，以 2%葡萄糖为碳源时，芽孢杆菌 CM27 的 PHA 产量最高，达到 32.1%，其他分离株的产量最高。优化培养基可使 Bacillus sp.CM27 的 CDW 的 PHA 产量（37.69%）增加。Bacillus sp.CM27 的氨基酸序列显示存在 PhaC 盒，序列为 G-Y-C-M-G-G，盒中间有半胱氨酸。通过傅里叶变换红外光谱法对提取的聚合物进行了确认。
