I. I. Mechnikov Research Institute of Vaccines and Sera, Moscow, Russia.
Department of Microbiology, Virology, and Immunology, I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.
Bull Exp Biol Med. 2022 Jul;173(3):354-360. doi: 10.1007/s10517-022-05548-6. Epub 2022 Jul 19.
We evaluated the effect of vaccination with anti-COVID-19 vaccine EpiVacCorona on serum antimicrobial activity, formation of specific IgG antibodies, and expression of some antimicrobial peptides. Antimicrobial activity of the serum from 55 volunteers towards S. aureus cells was measured spectrophotometrically; IgG-antibodies against SARS-CoV-2 antigen were assayed by ELISA; expression of genes encoding antimicrobial peptides LL37, HBD1, and HBD2 was evaluated by PCR with reverse transcription. Total antimicrobial serum activity and activity of its low-molecular-weight fraction containing antimicrobial peptides demonstrated an inverse correlation. Both activities after vaccination increased in case of low initial values, but decreased in case of high initial values. The vector of change of specific IgG antibodies to coronavirus inversely correlated with the vector of change of activity of antimicrobial peptide fraction. The expression of genes of antimicrobial peptides LL37, HBD1, and HBD2 looked like normal distribution depending on activities of the antimicrobial peptides in the corresponding sera.
我们评估了抗 COVID-19 疫苗 EpiVacCorona 接种对血清抗菌活性、特异性 IgG 抗体形成和某些抗菌肽表达的影响。通过分光光度法测量了 55 名志愿者血清对金黄色葡萄球菌细胞的抗菌活性；通过 ELISA 测定了针对 SARS-CoV-2 抗原的 IgG 抗体；通过逆转录 PCR 评估了编码抗菌肽 LL37、HBD1 和 HBD2 的基因的表达。总抗菌血清活性及其含有抗菌肽的低分子量部分的活性呈负相关。两种活性在初始值较低的情况下接种后均增加，但在初始值较高的情况下均降低。针对冠状病毒的特异性 IgG 抗体的变化向量与抗菌肽部分活性的变化向量呈反比。抗菌肽 LL37、HBD1 和 HBD2 的基因表达看起来呈正态分布，这取决于相应血清中抗菌肽的活性。
