Alatossava T, Jütte H, Seiler H
J Gen Virol. 1987 Jun;68 ( Pt 6):1525-32. doi: 10.1099/0022-1317-68-6-1525.
Phage LL-H-induced cation (K+, Na+, Mg2+, Ca2+, Cd2+) movements in Lactobacillus lactis bacteria have been studied. The effects of the m.o.i. and external cation concentration have been quantified. LL-H-induced effluxes showed cation specificity: K+ but practically no Mg2+ was lost during LL-H infection at low and moderate m.o.i. (up to about 100). Simultaneously to K+ efflux, divalent cation influxes were observed. These were dependent on the m.o.i. and on concentrations of external divalent cations and were concomitant with phage DNA transport, as concluded from the timing of the first phage-promoted biochemical changes in host cell metabolism and from electron microscopical observations. Host energy was not mobilized with phage-induced divalent cation influx. Several features of divalent cation influxes support the view that divalent cations have to be cotransported into the cell as counterions of LL-H DNA. Phage DNA associated with divalent cations may be the basic feature of the divalent cation dependence of LL-H infection.
对噬菌体LL-H诱导乳酸乳球菌中阳离子（K⁺、Na⁺、Mg²⁺、Ca²⁺、Cd²⁺）运动进行了研究。对感染复数（m.o.i.）和外部阳离子浓度的影响进行了量化。LL-H诱导的流出显示出阳离子特异性：在低和中等感染复数（高达约100）的LL-H感染期间，K⁺流失，但实际上没有Mg²⁺流失。在K⁺流出的同时，观察到二价阳离子流入。这些依赖于感染复数和外部二价阳离子的浓度，并且与噬菌体DNA转运同时发生，这是根据宿主细胞代谢中首次由噬菌体促进的生化变化的时间以及电子显微镜观察得出的结论。宿主能量不会随着噬菌体诱导的二价阳离子流入而调动。二价阳离子流入的几个特征支持这样一种观点，即二价阳离子必须作为LL-H DNA的抗衡离子共转运到细胞中。与二价阳离子相关的噬菌体DNA可能是LL-H感染对二价阳离子依赖性的基本特征。
