Alatossava T
J Gen Virol. 1982 Mar;59(Pt 1):173-5. doi: 10.1099/0022-1317-59-1-173.
Tris(hydroxymethyl)aminomethane (tris) inactivates Lactobacillus lactis bacteriophages LL-H in vitro. The inactivation is caused by DNA ejection. This effect occurs in tris-HCl buffer only. Both pH and temperature affect the sensitivity of the phage to the tris treatment. The divalent cation Mg2+ prevented the inactivating effected of tris at concentrations about 10(3)-fold lower than monovalent cation K+.
三（羟甲基）氨基甲烷（Tris）在体外可使乳酸乳球菌噬菌体LL-H失活。这种失活是由DNA排出引起的。此效应仅在Tris-HCl缓冲液中发生。pH值和温度都会影响噬菌体对Tris处理的敏感性。二价阳离子Mg2+在浓度比一价阳离子K+低约10³倍时就能阻止Tris的失活作用。
