College of Landscape Architecture and Tourism, Hebei Agricultural University, Baoding, P. R. China.
Department of Bioengineering, Hebei Agricultural University, Baoding, P. R. China.
PLoS One. 2022 Jul 19;17(7):e0271633. doi: 10.1371/journal.pone.0271633. eCollection 2022.
DNA barcoding is a supplementary tool in plant systematics that is extensively used to resolve species-level controversies. This study assesses the significance of using two DNA barcoding loci (e.g., psbA-trnH and trnC-petN) in distinguishing 33 plant samples of the genus Syringa. Results showed that the average genetic distance K2P of psbA-trnH DNA marker was 0.0521, which is much higher than that of trnC-petN, which is 0.0171. A neighbor-joining phylogenetic tree based on psbA-trnH and trnC-petN indicated that the identification rate of psbA-trnH and trnC-petN alone were 75% and 62.5%, respectively. The barcode combination of psbA-trnH+trnC-petN could identify 33 samples of the genus Syringa accurately and effectively with an identification rate of 87.5%. The 33 Syringa samples were divided into four groups: Group I is series Syringa represented by Syringa oblata; Group II is series Villosae represented by Syringa villosa; Group III is series Pubescentes represented by Syringa meyeri; and Group IV is section Ligustrina represented by Syringa reticulata subsp. pekinensis. These research results provided strong evidence that the combinatorial barcode of psbA-trnH+trnC-petN had high-efficiency identification ability and application prospects in species of the genus Syringa.
DNA 条形码是植物系统分类学中的一种辅助工具，广泛用于解决种级分类群的争议。本研究评估了使用两个 DNA 条形码基因座（如 psbA-trnH 和 trnC-petN）来区分丁香属的 33 个植物样本的重要性。结果表明，psbA-trnH DNA 标记的平均遗传距离 K2P 为 0.0521，明显高于 trnC-petN 的 0.0171。基于 psbA-trnH 和 trnC-petN 的邻接聚类系统发生树表明，psbA-trnH 和 trnC-petN 的单独鉴定率分别为 75%和 62.5%。psbA-trnH+trnC-petN 的组合条码能够准确有效地识别 33 个丁香属样本，鉴定率为 87.5%。这 33 个丁香属样本被分为四个组：第一组为以紫丁香为代表的丁香组；第二组为以暴马丁香为代表的暴马丁香组；第三组为以紫丁香为代表的毛丁香组；第四组为以北京丁香为代表的女贞组。这些研究结果为 psbA-trnH+trnC-petN 的组合条码在丁香属物种中的高效识别能力和应用前景提供了有力证据。
