Mol Biotechnol. 2013 Nov;55(3):249-59. doi: 10.1007/s12033-013-9676-2.
Chloroplast DNA has been used extensively to analyze plant phylogenies at different taxonomic levels because of its size, organization and sequence conservation. In the present research, two chloroplastic regions, petA–psaJ, trnC–trnD and four DNA barcodes (trnH–psbA, ITS, rbcL, matK), were used to introduce suitable regions for the assessment of genetic diversity among P. granatum L. genotypes. Analysis of psbE–petL in petA–psaJ region revealed 1,300 nucleotides with 4.29 % genetic diversity among genotypes, while trnC–petN in trnC–trnD region showed 1.8 % genetic diversity. Therefore, despite the results obtained from the study of other plants, the trnC–trnD region had a low potential for the evaluation of diversity among pomegranate genotypes. Analysis of DNA barcodes in pomegranate showed that trnH–psbA (genetic diversity 2.91 %) provides the highest intra-species variation, followed by ITS (genetic diversity 0.44 %). Eighteen genotypes from different geographical origins of Iran were used to investigate psbE–petL and trnH–psbA potential as novel barcodes to determine genetic polymorphism and characterize pomegranate genotypes. The results suggested that two regions, psbE–petL and trnH–psbA, were more suitable for determining intra-species relationships of pomegranate.
叶绿体 DNA 因其大小、组织和序列保守性，已被广泛用于分析不同分类水平的植物系统发育。在本研究中，使用了两个叶绿体区域（petA-psaJ、trnC-trnD）和四个 DNA 条形码（trnH-psbA、ITS、rbcL、matK），以引入适合评估石榴基因型遗传多样性的区域。在 petA-psaJ 区分析 psbE-petL 发现，基因型之间有 4.29%的遗传多样性，有 1300 个核苷酸；而在 trnC-trnD 区分析 trnC-petN 发现，有 1.8%的遗传多样性。因此，尽管从其他植物的研究中获得了结果，但 trnC-trnD 区对评估石榴基因型多样性的潜力较低。对石榴 DNA 条形码的分析表明，trnH-psbA（遗传多样性 2.91%）提供了最高的种内变异，其次是 ITS（遗传多样性 0.44%）。使用来自伊朗不同地理起源的 18 个基因型来研究 psbE-petL 和 trnH-psbA 作为新条形码的潜力，以确定遗传多态性并表征石榴基因型。结果表明，两个区域 psbE-petL 和 trnH-psbA 更适合确定石榴的种内关系。
