Department of Biology, Payame Noor University, Tehran, Iran.
Department of Biology, Payame Noor University, Tehran, Iran.
Food Chem. 2022 Dec 1;396:133711. doi: 10.1016/j.foodchem.2022.133711. Epub 2022 Jul 16.
Phytase is the commercial enzyme for bioconversion of phytate substrate to digestible phosphate ions. Recently silver nanoclusters (AgNCs) have received great attention as the optical transducer nanoparticles in biosensors structure. The novel detection platform was developed to detect the phytase enzyme activity and phosphate ions based on fluorescence quenching of AgNCs. The AgNCs were synthesized through gelatin supported reaction and characterized by TEM, FTIR and XRD analysis. The hydrolytic effect of phytase enzyme and subsequent phosphate release led to suppression of AgNCs fluorescence. The linear range was observed for enzyme in the range of 0.5-5 U/mL with the detection limit of 0.2 U/mL. Also, the same fluorescence quenching effect was observed in the presence of phosphate ion in the linear range of 1 to 16 µM with a detection limit of 0.5 µM. The proposed mechanism showed effectiveness of detection strategy for detection of phytase enzyme and phosphate ion.
植酸酶是将植酸盐底物生物转化为可消化的磷酸离子的商业酶。最近，银纳米簇（AgNCs）作为生物传感器结构中的光学转换纳米粒子受到了极大的关注。基于 AgNCs 的荧光猝灭，开发了一种新的检测平台来检测植酸酶活性和磷酸离子。AgNCs 通过明胶支撑的反应合成，并通过 TEM、FTIR 和 XRD 分析进行了表征。植酸酶酶的水解作用和随后的磷酸盐释放导致 AgNCs 荧光的抑制。在 0.5-5 U/mL 的范围内观察到酶的线性范围，检测限为 0.2 U/mL。此外，在 1 至 16 μM 的线性范围内观察到相同的荧光猝灭效应，检测限为 0.5 μM。所提出的机制表明，该检测策略可有效检测植酸酶和磷酸离子。
