Zhou Pei, Chen Bo, Hu Xinkai, Xiao Xiangyu, Liu Ruohan, Li Shoujun
Guangdong Provincial Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Guangdong Technological Engineering Research Center for Pets, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Guangdong Provincial Key Laboratory of Comprehensive Prevention and Control for Severe Clinical Animal Diseases, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China; Guangdong Technological Engineering Research Center for Pets, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Vet Microbiol. 2022 Sep;272:109501. doi: 10.1016/j.vetmic.2022.109501. Epub 2022 Jun 30.
At present, avian-origin H3N2 subtype canine influenza virus (H3N2 CIV) is prevalent in East Asian and North American countries. The host tropism of H3N2 CIV to mammals, including mice, guinea pigs, ferrets, and pigs, has been evaluated. However, it has not previously been determined whether avian-origin H3N2 CIV can be transmitted back to birds. In China, ducks, chickens, geese, pigeons, and quails are economically important domestic poultry that are susceptible to numerous subtypes of influenza A virus. These poultry occasionally or frequently come into contact with dogs. In this study, the infectivity of the first- and last-isolated Chinese H3N2 CIV strains in these poultry species was evaluated, and oropharyngeal and cloacal swabs of these animals were negative for virus, as determined by specific pathogen-free (SPF) chicken egg inoculation and real-time RT-qPCR assays. Clinical signs and gross lesions were not observed in any of these species, and seroconversion also did not occur. The results showed that all these avian species were unsusceptible to the first- and last-isolated H3N2 CIVs, indicating unidirectional evolution of the mammalian host tropism of H3N2 CIV.
目前，禽源H3N2亚型犬流感病毒（H3N2 CIV）在东亚和北美国家流行。H3N2 CIV对包括小鼠、豚鼠、雪貂和猪在内的哺乳动物的宿主嗜性已得到评估。然而，此前尚未确定禽源H3N2 CIV是否能传播回鸟类。在中国，鸭、鸡、鹅、鸽和鹌鹑是重要的经济家禽，易感染多种甲型流感病毒亚型。这些家禽偶尔或经常与狗接触。在本研究中，评估了首次分离和末次分离的中国H3N2 CIV毒株在这些家禽物种中的感染性，通过无特定病原体（SPF）鸡胚接种和实时RT-qPCR检测确定，这些动物的口咽和泄殖腔拭子病毒检测均为阴性。在这些物种中均未观察到临床症状和肉眼可见病变，也未发生血清转化。结果表明，所有这些禽类对首次分离和末次分离的H3N2 CIV均不敏感，表明H3N2 CIV的哺乳动物宿主嗜性呈单向进化。
