Lyoo Kwang-Soo, Kim Jeong-Ki, Kang Bokyu, Moon Hyoungjoon, Kim Jongman, Song Manki, Park Bongkyun, Kim Sang-Hyun, Webster Robert G, Song Daesub
Korea Zoonosis Research Institute, Chonbuk National University, Jeonju, Jeonbuk 561-756, Republic of Korea.
Department of Pharmacy, College of Pharmacy, Korea University, Sejong 339-700, Republic of Korea.
Virus Res. 2015 Jan 2;195:135-40. doi: 10.1016/j.virusres.2014.08.020. Epub 2014 Sep 16.
A novel avian-origin H3N2 canine influenza A virus (CIV) that showed high sequence similarities in hemagglutinin and neuraminidase genes with those of non-pathogenic avian influenza viruses was isolated in our routine surveillance program in South Korea. We previously reported that the pathogenicity of this strain could be reproduced in dogs and cats. In the present study, the host tropism of H3N2 CIV was examined by experimental inoculation into several host species, including chickens, pigs, mice, guinea pigs, and ferrets. The CIV infection resulted in no overt symptoms of disease in these host species. However, sero-conversion, virus shedding, and gross and histopathologic lung lesions were observed in guinea pig and ferrets but not in pigs, or mice. Based on the genetic similarity of our H3N2 CIV with currently circulating avian influenza viruses and the presence of α-2,3-linked rather than α-2,6-linked sialic acid receptors in the respiratory tract of dogs, we believed that this strain of CIV would have avian virus-like receptor specificity, but that seems to be contrary to our findings in the present study. Further studies are needed to determine the co-receptors of hemagglutinin or post-attachment factors related to virus internalization or pathogenesis in other animals.
在韩国的常规监测项目中，分离出了一种新型的源自禽类的H3N2犬流感病毒（CIV），该病毒的血凝素和神经氨酸酶基因与非致病性禽流感病毒的基因具有高度序列相似性。我们之前报道过，这种毒株的致病性在犬类和猫类中能够再现。在本研究中，通过将H3N2 CIV实验接种到包括鸡、猪、小鼠、豚鼠和雪貂在内的几种宿主物种中，来检测其宿主嗜性。CIV感染在这些宿主物种中未导致明显的疾病症状。然而，在豚鼠和雪貂中观察到了血清转化、病毒脱落以及大体和组织病理学肺部病变，但在猪和小鼠中未观察到。基于我们的H3N2 CIV与当前流行的禽流感病毒的基因相似性，以及犬类呼吸道中存在α-2,3连接而非α-2,6连接的唾液酸受体，我们认为这种CIV毒株具有禽流感病毒样的受体特异性，但这似乎与我们在本研究中的发现相悖。需要进一步研究来确定血凝素的共同受体或与病毒内化或发病机制相关的附着后因子在其他动物中的情况。
