• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

丁酸钠通过抑制 smad1 表达抑制人牙周膜干细胞成骨分化。

Sodium butyrate inhibits osteogenesis in human periodontal ligament stem cells by suppressing smad1 expression.

机构信息

Department of Orthodontics, School of Stomatology, Capital Medical University, Tian Tan Xi Li No.4, Beijing, 100050, People's Republic of China.

Laboratory of Tissue Regeneration and Immunology and Department of Periodontics, Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Capital Medical University, Beijing, People's Republic of China.

出版信息

BMC Oral Health. 2022 Jul 19;22(1):301. doi: 10.1186/s12903-022-02255-6.

DOI:10.1186/s12903-022-02255-6
PMID:35854293
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9297574/
Abstract

BACKGROUND

Butyrate is a major subgingival microbial metabolite that is closely related to periodontal disease. It affects the proliferation and differentiation of mesenchymal stem cells. However, the mechanisms by which butyrate affects the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) remain unclear. Here, we investigated the effect of sodium butyrate (NaB) on the osteogenic differentiation of human PDLSCs.

METHODS

PDLSCs were isolated from human periodontal ligaments and treated with various concentrations of NaB in vitro. The cell counting kit-8 assay and flow cytometric analysis were used to assess cell viability. The osteogenic differentiation capabilities of PDLSCs were evaluated using the alkaline phosphatase activity assay, alizarin red staining, RT-PCR, western blotting and in vivo transplantation.

RESULTS

NaB decreased PDLSC proliferation and induced apoptosis in a dose- and time-depend manner. Additionally, 1 mM NaB reduced alkaline phosphatase activity, mineralization ability, and the expression of osteogenic differentiation-related genes and proteins. Treatment with a free fatty acids receptor 2 (FFAR2) antagonist and agonist indicated that NaB inhibited the osteogenic differentiation capacity of PDLSCs by affecting the expression of Smad1.

CONCLUSION

Our findings suggest that NaB inhibits the osteogenic differentiation of PDLSCs by activating FFAR2 and decreasing the expression of Smad1.

摘要

背景

丁酸盐是一种与牙周病密切相关的主要龈下微生物代谢产物。它影响间充质干细胞的增殖和分化。然而，丁酸盐影响牙周韧带干细胞（PDLSCs）成骨分化的机制尚不清楚。在这里，我们研究了丁酸钠（NaB）对人牙周韧带干细胞（PDLSCs）成骨分化的影响。

方法

从人牙周韧带中分离出 PDLSCs，并在体外用不同浓度的 NaB 处理。细胞计数试剂盒-8 检测和流式细胞术分析用于评估细胞活力。通过碱性磷酸酶活性测定、茜素红染色、RT-PCR、western blot 和体内移植评估 PDLSCs 的成骨分化能力。

结果

NaB 呈剂量和时间依赖性地降低 PDLSC 增殖并诱导细胞凋亡。此外，1 mM NaB 降低碱性磷酸酶活性、矿化能力以及成骨分化相关基因和蛋白的表达。用游离脂肪酸受体 2（FFAR2）拮抗剂和激动剂处理表明，NaB 通过影响 Smad1 的表达来抑制 PDLSCs 的成骨分化能力。

结论

我们的研究结果表明，NaB 通过激活 FFAR2 和降低 Smad1 的表达来抑制 PDLSCs 的成骨分化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/b17cd494e837/12903_2022_2255_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/067d64d0a251/12903_2022_2255_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/ab72c9f075cf/12903_2022_2255_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/d1a995cc8fde/12903_2022_2255_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/8019037026ad/12903_2022_2255_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/b17cd494e837/12903_2022_2255_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/067d64d0a251/12903_2022_2255_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/ab72c9f075cf/12903_2022_2255_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/d1a995cc8fde/12903_2022_2255_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/8019037026ad/12903_2022_2255_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/81db/9297574/b17cd494e837/12903_2022_2255_Fig5_HTML.jpg

相似文献

1
Sodium butyrate inhibits osteogenesis in human periodontal ligament stem cells by suppressing smad1 expression.丁酸钠通过抑制 smad1 表达抑制人牙周膜干细胞成骨分化。
BMC Oral Health. 2022 Jul 19;22(1):301. doi: 10.1186/s12903-022-02255-6.
2
PAR1 Activation, via LMBR1/BMP Axis, Promotes the Osteogenesis of Periodontal Ligament Stem Cells (PDLSCs) and Alleviates the Inhibitory Effect of Sodium Butyrate on PDLSCs Osteogenesis.PAR1 通过 LMBR1/BMP 轴激活促进牙周膜干细胞（PDLSCs）成骨，并减轻丁酸钠对 PDLSCs 成骨的抑制作用。
Discov Med. 2024 Aug;36(187):1657-1671. doi: 10.24976/Discov.Med.202436187.152.
3
Circ_0003764 Regulates the Osteogenic Differentiation of Periodontal Ligament Stem Cells.环状 RNA 0003764 调控牙周膜干细胞的成骨分化。
Int Dent J. 2024 Oct;74(5):1110-1119. doi: 10.1016/j.identj.2024.03.004. Epub 2024 Mar 28.
4
Potassium dihydrogen phosphate promotes the proliferation and differentiation of human periodontal ligament stem cells via nuclear factor kappa B pathway.磷酸二氢钾通过核因子-κB 通路促进人牙周膜干细胞的增殖和分化。
Exp Cell Res. 2019 Nov 1;384(1):111593. doi: 10.1016/j.yexcr.2019.111593. Epub 2019 Sep 2.
5
Thyrotropin inhibits osteogenic differentiation of human periodontal ligament stem cells.促甲状腺激素抑制人牙周膜干细胞的成骨分化。
J Periodontal Res. 2023 Jun;58(3):668-678. doi: 10.1111/jre.13109. Epub 2023 Feb 20.
6
[Rutin promotes osteogenic differentiation of periodontal ligament stem cells under inflammatory microenvironment].[芦丁促进炎症微环境下牙周膜干细胞的成骨分化]
Shanghai Kou Qiang Yi Xue. 2019 Aug;28(4):356-361.
7
[The effect of Toll-like receptor 4 in nicotine suppressing the osteogenic potential of periodontal ligament stem cells].[Toll样受体4在尼古丁抑制牙周膜干细胞成骨潜能中的作用]
Hua Xi Kou Qiang Yi Xue Za Zhi. 2017 Aug 1;35(4):368-372. doi: 10.7518/hxkq.2017.04.005.
8
All-trans retinoic acid inhibits the osteogenesis of periodontal ligament stem cells by promoting IL-1β production via NF-κB signaling.全反式维甲酸通过 NF-κB 信号通路促进白细胞介素-1β的产生，从而抑制牙周膜干细胞的成骨分化。
Int Immunopharmacol. 2022 Jul;108:108757. doi: 10.1016/j.intimp.2022.108757. Epub 2022 Apr 15.
9
Sequential application of bFGF and BMP-2 facilitates osteogenic differentiation of human periodontal ligament stem cells.碱性成纤维细胞生长因子和骨形态发生蛋白-2 的顺序应用促进人牙周膜干细胞的成骨分化。
J Periodontal Res. 2019 Aug;54(4):424-434. doi: 10.1111/jre.12644. Epub 2019 Mar 9.
10
Enamel matrix derivative enhances the proliferation and osteogenic differentiation of human periodontal ligament stem cells on the titanium implant surface.釉基质衍生物增强人牙周膜干细胞在钛种植体表面的增殖和成骨分化。
Organogenesis. 2017 Jul 3;13(3):103-113. doi: 10.1080/15476278.2017.1331196. Epub 2017 Jun 9.

引用本文的文献

1
Metabolic Footprint of and Reveals Potential Interaction Towards Community Succession and Pathogenesis in Bovine Digital Dermatitis.金黄色葡萄球菌和产黑色素普雷沃菌的代谢足迹揭示了其在牛趾间皮炎中对群落演替和发病机制的潜在相互作用。
Pathogens. 2024 Sep 14;13(9):796. doi: 10.3390/pathogens13090796.
2
[Relationship between short-chain fatty acids in the gingival crevicular fluid and periodontitis of stage Ⅲ or Ⅳ].龈沟液中短链脂肪酸与Ⅲ或Ⅳ期牙周炎的关系
Beijing Da Xue Xue Bao Yi Xue Ban. 2024 Apr 18;56(2):332-337. doi: 10.19723/j.issn.1671-167X.2024.02.021.
3
Butyrate attenuates the stemness of lung cancer cells through lysosome Fe- and SLC7A11-mediated ferroptosis.

本文引用的文献

1
Single-Cell Atlas Reveals Fatty Acid Metabolites Regulate the Functional Heterogeneity of Mesenchymal Stem Cells.单细胞图谱揭示脂肪酸代谢物调节间充质干细胞的功能异质性。
Front Cell Dev Biol. 2021 Apr 12;9:653308. doi: 10.3389/fcell.2021.653308. eCollection 2021.
2
Microbial Regulation of Host Physiology by Short-chain Fatty Acids.短链脂肪酸对宿主生理学的微生物调节作用。
Trends Microbiol. 2021 Aug;29(8):700-712. doi: 10.1016/j.tim.2021.02.001. Epub 2021 Mar 2.
3
Local and systemic mechanisms linking periodontal disease and inflammatory comorbidities.
丁酸通过溶酶体铁和溶质载体家族7成员11（SLC7A11）介导的铁死亡减弱肺癌细胞的干性。
Heliyon. 2024 Mar 13;10(7):e28093. doi: 10.1016/j.heliyon.2024.e28093. eCollection 2024 Apr 15.
4
The Experimental Study of Periodontal Ligament Stem Cells Derived Exosomes with Hydrogel Accelerating Bone Regeneration on Alveolar Bone Defect.牙周膜干细胞来源的外泌体与水凝胶促进牙槽骨缺损骨再生的实验研究
Pharmaceutics. 2022 Oct 14;14(10):2189. doi: 10.3390/pharmaceutics14102189.
牙周病与炎症性合并症相关的局部和全身机制。
Nat Rev Immunol. 2021 Jul;21(7):426-440. doi: 10.1038/s41577-020-00488-6. Epub 2021 Jan 28.
4
CTP-CM enhances osteogenic differentiation of hPDLSCs via NF-κB pathway.CTP-CM 通过 NF-κB 通路增强 hPDLSCs 的成骨分化。
Oral Dis. 2021 Apr;27(3):577-588. doi: 10.1111/odi.13567. Epub 2020 Aug 31.
5
Microbiota-Derived Short-Chain Fatty Acids Promote BMP Signaling by Inhibiting Histone Deacetylation and Contribute to Dentinogenic Differentiation in Murine Incisor Regeneration.微生物衍生的短链脂肪酸通过抑制组蛋白去乙酰化促进 BMP 信号传导，并有助于小鼠切牙再生中的牙本质发生分化。
Stem Cells Dev. 2020 Sep 15;29(18):1201-1214. doi: 10.1089/scd.2020.0057. Epub 2020 Aug 17.
6
Butyrate Decreases ICAM-1 Expression in Human Oral Squamous Cell Carcinoma Cells.丁酸盐可降低人口腔鳞状细胞癌细胞中 ICAM-1 的表达。
Int J Mol Sci. 2020 Feb 29;21(5):1679. doi: 10.3390/ijms21051679.
7
Free Fatty Acid Receptors in Health and Disease.游离脂肪酸受体在健康与疾病中的作用
Physiol Rev. 2020 Jan 1;100(1):171-210. doi: 10.1152/physrev.00041.2018. Epub 2019 Sep 5.
8
Butyrate rather than LPS subverts gingival epithelial homeostasis by downregulation of intercellular junctions and triggering pyroptosis.丁酸盐通过下调细胞间连接并触发细胞焦亡从而破坏牙龈上皮细胞的稳态，而不是 LPS。
J Clin Periodontol. 2019 Sep;46(9):894-907. doi: 10.1111/jcpe.13162.
9
Short-chain fatty acids and FFAR2 as suppressors of bone resorption.短链脂肪酸和 FFAR2 作为抑制骨吸收的物质。
Bone. 2019 Aug;125:112-121. doi: 10.1016/j.bone.2019.05.016. Epub 2019 May 14.
10
The oral microbiota: dynamic communities and host interactions.口腔微生物组：动态群落与宿主相互作用。
Nat Rev Microbiol. 2018 Dec;16(12):745-759. doi: 10.1038/s41579-018-0089-x.