Kawano J, Ney D M, Keen C L, Schneeman B O
J Nutr. 1987 May;117(5):902-6. doi: 10.1093/jn/117.5.902.
The objective of this experiment was to determine whether changes in liver cholesterol content, plasma lipids and lipoprotein composition result from dietary manganese (Mn) deficiency in the Sprague-Dawley (SD) and the Wistar (W) rat. Weanling rats of both strains were fed Mn-deficient (1 ppm Mn) or Mn-adequate (45 ppm Mn) diets for 10 wk. After the 10-wk period rats were killed and plasma, liver, kidney and heart were collected. Liver, kidney and heart Mn concentrations were significantly lower in the rats fed the Mn-deficient diet than in controls. Plasma high density lipoprotein (HDL) (density 1.050-1.1963) was isolated by sequential ultracentrifugation. Plasma cholesterol levels were similar for all groups. HDL protein, cholesterol and apo E levels were significantly reduced with Mn deficiency. A shift to a smaller HDL particle was associated with Mn deficiency, based on gradient gel electrophoresis. The shift in size was more pronounced in the SD strain. Liver concentrations of total and free cholesterol were lower with Mn deficiency but more noticeably in the SD group. The results of our study suggest a role for Mn in HDL metabolism in vivo in both SD and W rats with a trend toward a greater effect in the SD strain.
本实验的目的是确定在斯普拉格-道利（SD）大鼠和Wistar（W）大鼠中，肝脏胆固醇含量、血浆脂质和脂蛋白组成的变化是否由膳食锰（Mn）缺乏引起。两种品系的断奶大鼠分别喂食缺锰（1 ppm锰）或锰充足（45 ppm锰）的饲料10周。10周后处死大鼠，收集血浆、肝脏、肾脏和心脏。喂食缺锰饲料的大鼠肝脏、肾脏和心脏中的锰浓度显著低于对照组。通过连续超速离心分离血浆高密度脂蛋白（HDL）（密度1.050 - 1.1963）。所有组的血浆胆固醇水平相似。缺锰会使HDL蛋白、胆固醇和载脂蛋白E水平显著降低。基于梯度凝胶电泳，缺锰与HDL颗粒向较小尺寸的转变有关。这种尺寸变化在SD品系中更为明显。缺锰时肝脏中总胆固醇和游离胆固醇的浓度较低，但在SD组中更明显。我们的研究结果表明，锰在SD和W大鼠体内HDL代谢中发挥作用，且在SD品系中有产生更大影响的趋势。
