Comprehensive identification, evolutionary patterns and the divergent response of genes in under biotic and abiotic interactions.

UNLABELLED

Peroxiredoxins (Prxs) are novel cysteine-based peroxidases which are involved in protecting cells from oxidative damage by catalyzing the reduction of different peroxides. The present study addressed, for the first time, genome-wide identification, evolutionary patterns and expression dynamics of gene family (). Nine Prx proteins were identified in based on homology searches. The phylogeny analysis of Prxs from seven plant species revealed that Prx proteins can be clustered into four groups (1C-Prx, 2C-Prxs, PrxQ and type II Prxs). Both tandem and segmental duplication contributed to gene family expansion. Intragenic reorganizations including gain/loss of exon/intron and insertions/deletions have also contributed to gene diversification. The collinearity analysis revealed the presence of some orthologous gene pairs between and genomes. The Ka/Ks ratio indicated that two of the three duplicated gene pairs have undergone a purifying selection. Redundant stress-related cis-acting elements were also found in the promoters of most genes. RT q-PCR analysis revealed an upregulation of key members in response to symbiosis and different abiotic factors. The upregulation of targeted members, particularly in leaves exposed to salinity or drought, was accompanied by an accumulation of hydrogen peroxide (HO). When exogenously applied, HO modulated almost all genes, suggesting a potential HO-scavenging role for these proteins. Collectively, our analysis provided valuable information for further functional analysis of key members to improve common bean stress tolerance and/or its symbiotic performance.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s13205-022-03246-8.