Liang Xinyu, Li Yongming, Chu Pengfei, Wang Qian, Wang Hanyue, Liao Lanjie, Yang Cheng, Zhu Zuoyan, Wang Yaping, He Libo
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Antioxidants (Basel). 2022 Sep 29;11(10):1952. doi: 10.3390/antiox11101952.
Peroxiredoxins are a family of antioxidant proteins that protect cells from oxidative damage caused by reactive oxygen species (ROS). Herein, the peroxiredoxin 3 gene from grass carp (), named , was cloned and analyzed. The full-length cDNA of is 1068 bp long, with a 753 bp open reading frame (ORF) that contains a thioredoxin-2 domain, two peroxiredoxin signature motifs, and two highly conserved cysteine residues. was ubiquitously expressed in all the tested tissues, while its expression level was altered significantly after exposure to grass carp reovirus (GCRV) and pathogen-associated molecular patterns (PAMPs). was localized in the mitochondria of transfected cells and concentrated in the nucleus after poly (I:C) treatment. Transformation of into enhanced host resistance to HO and heavy metals. Purified recombinant proteins could protect DNA against oxidative damage. Overexpression of in fish cells reduced intracellular ROS, increased cell viability, and decreased cell apoptosis caused by HO stimulation and GCRV infection. Further study indicated that induced autophagy to inhibit GCRV replication in fish cells. Collectively, these results imply that grass carp Prx3 elevates host antioxidant activity and induces autophagy to inhibit GCRV replication.
过氧化物酶体增殖物激活受体是一类抗氧化蛋白家族，可保护细胞免受活性氧（ROS）引起的氧化损伤。在此，克隆并分析了草鱼的过氧化物酶体增殖物激活受体3基因（命名为）。的全长cDNA长1068 bp，具有753 bp的开放阅读框（ORF），其中包含一个硫氧还蛋白-2结构域、两个过氧化物酶体增殖物激活受体特征基序和两个高度保守的半胱氨酸残基。在所有测试组织中均广泛表达，而在暴露于草鱼呼肠孤病毒（GCRV）和病原体相关分子模式（PAMPs）后，其表达水平发生了显著变化。在转染细胞的线粒体中定位，并在聚（I:C）处理后集中在细胞核中。将转化为可增强宿主对HO和重金属的抗性。纯化的重组蛋白可以保护DNA免受氧化损伤。在鱼细胞中过表达可降低细胞内ROS，提高细胞活力，并减少由HO刺激和GCRV感染引起的细胞凋亡。进一步研究表明，诱导自噬以抑制鱼细胞中GCRV的复制。总体而言，这些结果表明草鱼Prx3提高了宿主抗氧化活性并诱导自噬以抑制GCRV复制。
