Medberry Ava N, Srivastava Ashish, Diaz-Lara Alfredo, Rwahnih Maher Al, Villamor Dan E V, Tzanetakis Ioannis E
Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701, U.S.A.
Amity Institute of Virology & Immunology, Amity University Uttar Pradesh, Sector 125, Noida, UP 284403, India.
Plant Dis. 2023 Mar;107(3):620-623. doi: 10.1094/PDIS-05-22-1078-SC. Epub 2023 Mar 1.
Strawberry ( × ) is the most important berry crop worldwide and viruses pose a constant threat to the industry. In this communication, we describe a novel virus in the family referred to as strawberry virus 3 (StrV-3). The virus does not show significant homology when compared with recognized rhabdoviruses and, therefore, the establishment of a new genus should be considered. A triplex reverse-transcription PCR test was developed and successfully employed in a survey of the National Clonal Germplasm Repository collection. A CRISPR-Cas-based protocol was also developed and shown to detect the virus in as little as 1 fg of total RNA, a protocol to be used in the detection of the virus in candidate G1 plants. The strawberry aphid () was evaluated-alas, unsuccessfully-as a potential vector of the virus. This work broadens our understanding of the family and assists in the quest of releasing plant material free of viruses.
草莓（×）是全球最重要的浆果作物，病毒对该产业构成持续威胁。在本通讯中，我们描述了一种属于该科的新型病毒，称为草莓病毒3（StrV-3）。与公认的弹状病毒相比，该病毒没有显著的同源性，因此应考虑建立一个新属。开发了一种三重逆转录PCR检测方法，并成功应用于国家克隆种质资源库的调查收集。还开发了一种基于CRISPR-Cas的方案，该方案显示能够在低至1 fg的总RNA中检测到该病毒，该方案将用于检测候选G1植物中的病毒。对草莓蚜（）作为该病毒的潜在传播媒介进行了评估——遗憾的是未成功。这项工作拓宽了我们对该科的认识，并有助于培育无病毒植物材料。
