Institute of Plant Molecular Biology, Department of Plant Virology, Biology Centre CAS, 370 05 Ceske Budejovice, Czech Republic.
Faculty of Agriculture, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic.
Viruses. 2022 Jun 23;14(7):1362. doi: 10.3390/v14071362.
Advances in high-throughput sequencing methods have boosted the discovery of multistrain viral infections in diverse plant systems. This phenomenon appears to be pervasive for certain viral species. However, our knowledge of the transmission aspects leading to the establishment of such mixed infections is limited. Recently, we reported a mixed infection of a single strawberry plant with strawberry mottle virus (SMoV), strawberry crinkle virus (SCV) and strawberry virus 1 (StrV-1). While SCV and StrV-1 are represented by two and three molecular variants, respectively, SmoV has three different RNA1 and RNA2 segments. In this study, we focus on virus acquisition by individual adult aphids of the , and species. Single-aphid transmission trials are performed under experimental conditions. Both different viruses and individual virus strains show varying performances in single aphid acquisition. The obtained data suggests that numerous individual transmission events lead to the establishment of multistrain infections. These data will be important for the development of epidemiological models in plant virology.
高通量测序方法的进步推动了在不同植物系统中发现多株病毒感染。对于某些病毒种类来说，这种现象似乎很普遍。然而，我们对于导致这种混合感染建立的传播方面的了解是有限的。最近，我们报道了单一草莓植株同时感染草莓斑驳病毒（SMoV）、草莓皱缩病毒（SCV）和草莓病毒 1（StrV-1）的情况。虽然 SCV 和 StrV-1 分别由两个和三个分子变体代表，但 SmoV 有三个不同的 RNA1 和 RNA2 片段。在这项研究中，我们专注于个体成年蚜虫对 、 和 物种的病毒获取。在实验条件下进行单只蚜虫传播试验。不同的病毒和单个病毒株在单个蚜虫获取中表现出不同的性能。获得的数据表明，许多单独的传播事件导致了多株感染的建立。这些数据对于植物病毒学中流行病学模型的发展将是重要的。
