乙型肝炎表面抗原（HBsAg）的夹心酶免疫测定法。
Sandwich enzymoimmunoassay of hepatitis B surface antigen (HBsAg).
作者信息
Adachi H, Fukuda T, Funahashi S, Kurahori T, Ishikawa E
出版信息
Vox Sang. 1978;35(4):219-23. doi: 10.1111/j.1423-0410.1978.tb02925.x.
A sandwich enzymoimmunoassay (EIA) procedure was developed for the detection of HBsAg using Fab' of anti-HBsAg conjugated with beta-D-galactosidase from Escherichia coli with anti-HBsAg-coated silicone rubber discs as a solid phase. EIA could detect 1 ng/ml of HBsAg. It was as sensitive as radioimmunoassay (RIA, AusRia II) and about 30-fold more sensitive than reversed passive hemagglutination assay RPHA, ReverseCell). EIA and RIA could detect more HBsAg-positive sera than RPHA.
开发了一种夹心酶免疫分析（EIA）方法，以抗HBsAg的Fab'与来自大肠杆菌的β-D-半乳糖苷酶偶联物检测HBsAg，以抗HBsAg包被的硅橡胶盘作为固相。EIA能够检测到1 ng/ml的HBsAg。其灵敏度与放射免疫分析（RIA，AusRia II）相当，比反向被动血凝试验（RPHA，ReverseCell）灵敏约30倍。EIA和RIA能够检测出比RPHA更多的HBsAg阳性血清。
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