使用商业放射免疫分析试剂改进乙肝表面抗原筛查的经济性。
Improved economics of HBsAg screening with commercial radioimmunoassay reagents.
作者信息
Hopkins R, Ross S, Jordan T, Watt A D
出版信息
J Clin Pathol. 1980 Jan;33(1):19-23. doi: 10.1136/jcp.33.1.19.
Abstract
Commercial 125I anti-HBs was processed to yield a sixfold improvement in economy without significant loss of sensitivity or specificity. Additional polystyrene beads were coupled with commercially supplied anti-HBs. The modified assay (Mod-RIA) was compared with commercial RIA, EIA, and RPHA using established HBsAg panels. Mod-RIA was also compared with HEPATEST (RPHA) for screening 71 200 fresh blood donations during an 11.5-month period.
摘要
对市售的125I抗-HBs进行处理，使其经济性提高了六倍，且灵敏度和特异性没有明显损失。将额外的聚苯乙烯珠与市售抗-HBs偶联。使用已建立的HBsAg检测板，将改良检测法（Mod-RIA）与市售RIA、EIA和RPHA进行比较。在11.5个月的时间里，还将Mod-RIA与HEPATEST（RPHA）进行比较，用于筛查71200份新鲜血液捐献样本。
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