Weissert Robert, Hugosson Therése, Petzold Axel
Department of Neurology, University Hospital of Regensburg, Regensburg, Germany.
Department of Ophthalmology, St Erik Eye Hospital, Stockholm, Sweden.
Neuroophthalmology. 2022 Jan 26;46(4):215-219. doi: 10.1080/01658107.2022.2025852. eCollection 2022.
In optic neuritis (ON), transient thickening of the macular retinal nerve fibre layer (RNFL) can be observed. This optical coherence tomography-based observation is not understood. The axonal diameter correlates with the neurofilament (Nf) protein content, but there are no data on the retinal tissue concentration of Nfs. The myelin-oligodendrocyte-glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) model was used to investigate the retinas of Brown Norway rats with (i) visual evoked potentials (VEP) confirmed ON, (ii) VEP confirmed absence of ON and (iii) control animals. Twenty retinas were collected from MOG-EAE and control rats 27 days after immunisation. Retinal tissue Nf concentrations per total protein (g/mg) were significantly higher in MOG-EAE rats with ON (median 4.29, interquartile range [IQR] 3.41-5.97) compared with MOG-EAE rats without ON (1.14, IQR 1.10-1.67) or control rats (0.93, IQR 0.45-4.00). The data suggest that up-regulation of Nf expression in the retinal ganglion cells precedes development of RNFL atrophy and plausibly explains the transient increase of axonal diameter and RNFL thickening.
在视神经炎（ON）中，可以观察到黄斑视网膜神经纤维层（RNFL）的短暂增厚。基于光学相干断层扫描的这一观察结果尚不清楚。轴突直径与神经丝（Nf）蛋白含量相关，但尚无关于视网膜组织中Nf浓度的数据。采用髓鞘少突胶质细胞糖蛋白（MOG）诱导的实验性自身免疫性脑脊髓炎（EAE）模型，对（i）视觉诱发电位（VEP）证实患有ON的、（ii）VEP证实未患ON的以及（iii）对照的棕色挪威大鼠视网膜进行研究。免疫27天后，从MOG-EAE大鼠和对照大鼠中收集了20个视网膜。与未患ON的MOG-EAE大鼠（中位数1.14，四分位间距[IQR] 1.10 - 1.67）或对照大鼠（0.93，IQR 0.45 - 4.00）相比，患ON的MOG-EAE大鼠视网膜组织中每总蛋白（g/mg）的Nf浓度显著更高（中位数4.29，IQR 3.41 - 5.97）。数据表明，视网膜神经节细胞中Nf表达上调先于RNFL萎缩的发生，并且合理地解释了轴突直径的短暂增加和RNFL增厚。
