Miller D L, Lamore B J
J Ultrasound Med. 1987 May;6(5):231-6. doi: 10.7863/jum.1987.6.5.231.
Antigen release from human type B red blood cells was investigated by sensitive capillary-tube assay techniques after subjecting 3% suspensions to a controlled form of ultrasonic cavitation. Cells were kept suspended during the 1,000-sec exposures by an orbital motion of the sample chamber. Several spatial peak intensities of continuous 1.7 MHz ultrasound were applied to the samples which included gas-filled 4-microns diameter micropores in hydrophobic membranes. Free hemoglobin, indicating hemolysis, and free antigenic material, indicating solubilization of antigens, were found in the supernates of suspensions exposed to 90 mW/cm2 or 180 mW/cm2, respectively, or greater intensities. None of the free antigenic material could be attributed to antigen loss by surviving cells. For these conditions, the antigen release effect of ultrasound appears to be a byproduct of cavitation-induced cell lysis.
采用灵敏的毛细管检测技术，在对3%的悬浮液进行可控形式的超声空化处理后，研究了B型人红细胞的抗原释放情况。在1000秒的暴露过程中，通过样品室的圆周运动使细胞保持悬浮状态。将连续1.7MHz超声的几个空间峰值强度应用于样品，这些样品包括疏水膜中直径为4微米的充气微孔。在分别暴露于90mW/cm²或180mW/cm²或更高强度的悬浮液上清液中，发现了表明溶血的游离血红蛋白和表明抗原溶解的游离抗原物质。没有任何游离抗原物质可归因于存活细胞的抗原损失。在这些条件下，超声的抗原释放效应似乎是空化诱导细胞裂解的副产物。
