Miller D L, Williams A R
Ultrasound Med Biol. 1983 May-Jun;9(3):297-307. doi: 10.1016/0301-5629(83)90063-7.
Suspensions of human erythrocytes in a firefly-extract solution were exposed to 2.0 MHz ultrasound in the presence of 3.4 microns dia. gas-filled pores. Lysis of the cells during ultrasonic exposure resulted in the release of ATP which produced light upon interaction with the firefly extract. The response was approximately proportional to the square of the SPTA intensity I for both continuous and pulsed exposures (1 mu s pulses, 1:9 duty cycle) within the range 5-100 mW/cm2. One method of graphical data presentation yielded an apparent threshold for the effect at 0.31 bar pressure amplitude; however, a response could probably be obtained at any nonzero exposure level with a sufficiently sensitive apparatus. The response was also proportional to the exposure duration t for the range 0.03-3 s. The product I2 t may have some significance as a dosage parameter for this system in an, as yet, undefined "low-dose" range. Measurements for exposures with higher intensities, longer durations, lower duty factors and with pulses longer than 300 mu s deviated from these trends.
将人红细胞悬浮液置于含有直径为3.4微米充气孔隙的萤火虫提取物溶液中，在2.0兆赫超声作用下进行处理。超声照射期间细胞裂解导致ATP释放，ATP与萤火虫提取物相互作用时会发光。对于连续和脉冲照射（1微秒脉冲，1:9占空比），在5 - 100毫瓦/平方厘米范围内，响应大致与SPTA强度I的平方成正比。一种图形数据呈现方法得出该效应的表观阈值为0.31巴压力幅值；然而，使用足够灵敏的仪器，在任何非零照射水平下可能都能获得响应。在0.03 - 3秒范围内，响应也与照射持续时间t成正比。在尚未明确的“低剂量”范围内，乘积I²t作为该系统的剂量参数可能具有一定意义。对于更高强度、更长持续时间、更低占空比以及脉冲长于300微秒的照射测量结果偏离了这些趋势。
