Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Tokyo, Japan.
Ehime Fisheries Research Center, Ehime Research Institute of Agriculture, Forestry and Fisheries, Ehime, Japan.
J Fish Dis. 2022 Oct;45(10):1593-1597. doi: 10.1111/jfd.13690. Epub 2022 Jul 21.
Red sea bream iridovirus (RSIV) is the pathogen that causes red sea bream iridoviral disease. It causes a huge loss to the Japanese aquaculture industry. In 2021, outbreaks of red sea bream iridovirus occurred in South Japan. This study analysed nine whole-genome sequences of RSIV isolated in Oita and Ehime Prefectures in 2021 using a short-read next-generation sequencer. Nine isolates had highly uniform sequences, and there was no variant depending on locations or host species. Phylogenetic analyses with other reported megalocytivirus isolates showed that RSIV isolated in 2021 was genetically different from RSIV previously isolated in Oita and Ehime Prefectures in 2017-2019. These results suggest that RSIV isolated in Oita and Ehime Prefectures in 2021 might spread from a common ancestor different from the recent one. Additionally, it was found that RSIV isolated in 2021 had sequence mutations on protein-coding sequences that may be involved in viral pathogenicity and infectivity.
真鲷虹彩病毒（RSIV）是引起真鲷虹彩病毒病的病原体，它给日本水产养殖业造成了巨大损失。2021 年，日本南部地区爆发了真鲷虹彩病毒病。本研究使用短读长下一代测序仪，对 2021 年在大分县和爱媛县分离到的 9 株 RSIV 全基因组序列进行了分析。9 株分离株具有高度一致的序列，不存在因地理位置或宿主种类而异的变异。与其他已报道的巨细胞病毒分离株的系统进化分析表明，2021 年分离到的 RSIV 在遗传上与 2017-2019 年在大分县和爱媛县分离到的 RSIV 不同。这些结果表明，2021 年在大分县和爱媛县分离到的 RSIV 可能是由不同于最近的共同祖先传播而来。此外，还发现 2021 年分离到的 RSIV 在编码蛋白的序列上发生了突变，这些突变可能与病毒的致病性和感染力有关。
