Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Parkville, Australia.
Mol Oral Microbiol. 2023 Feb;38(1):34-40. doi: 10.1111/omi.12383. Epub 2022 Aug 3.
Porphyromonas gingivalis is an anaerobic Gram-negative human oral pathogen highly associated with the more severe forms of periodontal disease. Porphyromonas gingivalis utilises the type IX secretion system (T9SS) to transport ∼30 cargo proteins, including multiple virulence factors, to the cell surface. The T9SS is a multiprotein system consisting of at least 20 proteins, and recently, we characterised the protein interactome of these components. Similar to the T9SS, almost all biological processes are mediated through protein-protein interactions (PPIs). Therefore, mapping PPIs is important to understand the biological functions of many proteins in P. gingivalis. Herein, we provide native migration profiles of over 1000 P. gingivalis proteins. Using the T9SS, we demonstrate that our dataset is a useful resource for identifying novel protein interactions. Using this dataset and further analysis of T9SS P. gingivalis mutants, we discover new mechanistic insights into the formation of the PorQ-Z complex of the T9SS. This dataset is a valuable resource for studies of P. gingivalis.
牙龈卟啉单胞菌是一种厌氧革兰氏阴性人类口腔病原体，与牙周病的更严重形式密切相关。牙龈卟啉单胞菌利用九型分泌系统（T9SS）将约 30 种货物蛋白，包括多种毒力因子，运送到细胞表面。T9SS 是一个由至少 20 种蛋白组成的多蛋白系统，最近，我们对这些成分的蛋白互作组进行了表征。与 T9SS 类似，几乎所有的生物过程都是通过蛋白-蛋白相互作用（PPIs）介导的。因此，绘制 PPI 图谱对于理解许多蛋白质在牙龈卟啉单胞菌中的生物学功能非常重要。本文中，我们提供了超过 1000 种牙龈卟啉单胞菌蛋白的天然迁移图谱。利用 T9SS，我们证明了我们的数据集是识别新蛋白相互作用的有用资源。利用这个数据集和对 T9SS 牙龈卟啉单胞菌突变体的进一步分析，我们发现了 T9SS 中 PorQ-Z 复合物形成的新的机制见解。这个数据集是研究牙龈卟啉单胞菌的有价值的资源。
