Laboratoire des Multimatériaux et Interfaces, UMR CNRS 5615, Univ Lyon - Claude Bernard Lyon 1, Villeurbanne, France.
Faculté d'Odontologie, Univ Lyon - Claude Bernard Lyon 1, Lyon, France.
Int Endod J. 2022 Oct;55(10):1053-1065. doi: 10.1111/iej.13803. Epub 2022 Aug 4.
To assess in vitro the effect of two novel phase separated borosilicate glasses (PSBS) in the system SiO -B O -K O-CaO-Al O on dental pulp cells; and to compare their bioactivity and mechanical properties to a conventional fluoroaluminosilicate glass ionomer cement namely FUJI IX.
The cytocompatibility assessment of the two novel borosilicate glasses, one without alumina (PSBS8) and one containing alumina (PSBS16), was performed on cultured primary human pulp cells. Alamar blue assay was used to assess cell metabolic activity and cell morphology was evaluated by confocal imaging. The bioactivity in Stimulated Body Fluid was also evaluated after 1 and 3 weeks of immersion using SEM-EDX analysis. Vickers microhardness and flexural strength were assessed after incorporating the glass particles into a commercial glass ionomer cement (GIC) liquid containing both polyacrylic and polybasic carboxylic acid.
The data revealed that the two borosilicate glasses enhanced cell viability ratios at all-time points in both direct and indirect contact assays. After 3 days of contact, PSBS8 without alumina showed higher viability rate (152%) compared to the PSBS16 containing alumina (145%) and the conventional glass ionomer particles (117%). EDX analysis confirmed an initial Ca/P ratio of 2.1 for 45S5K and 2.08 for PSBS8 without alumina after 3 weeks of immersion. The cement prepared using PSBS8 showed significantly higher Vickers hardness values (p = .001) than that prepared using PSBS16 (46.6 vs. 36.7 MPa). After 24 h of maturation, PSBS8 (without alumina) exhibited a flexural strength of 12.9 MPa compared to a value of 16.4 MPa for the commercial control. PSBS8 without alumina had a higher strength than PSBS16 with alumina, after 1 and 7 days of maturation (p = .001).
The present in vitro results demonstrated that the borosilicate bioactive glass without alumina enhanced pulp cell viability, spreading and acellular bioactivity better than the conventional GIC and the experimental borosilicate glass containing alumina.
评估两种新型相分离硼硅酸盐玻璃（PSBS）在 SiO-B O-K O-CaO-Al O 系统中对牙髓细胞的体外影响；并将其生物活性和机械性能与传统的氟铝硅酸盐玻璃离子水门汀 Fuji IX 进行比较。
对两种新型硼硅酸盐玻璃（一种不含氧化铝 PSBS8 和一种含氧化铝 PSBS16）进行细胞相容性评估，方法是在培养的原代人牙髓细胞上进行。使用阿尔玛蓝测定法评估细胞代谢活性，并通过共聚焦成像评估细胞形态。在浸泡 1 周和 3 周后，还使用 SEM-EDX 分析评估了在刺激体液中的生物活性。将玻璃颗粒掺入含有聚丙烯酸和多羧酸的商业玻璃离子水门汀（GIC）液体中后，评估维氏硬度和弯曲强度。
数据显示，两种硼硅酸盐玻璃在直接和间接接触试验中均在所有时间点提高了细胞活力比率。接触 3 天后，不含氧化铝的 PSBS8 显示出比含氧化铝的 PSBS16（145%）和传统玻璃离子体颗粒（117%）更高的活力率（152%）。EDX 分析证实，在浸泡 3 周后，45S5K 的初始 Ca/P 比为 2.1，PSBS8 不含氧化铝为 2.08。使用 PSBS8 制备的水泥的维氏硬度值明显高于使用 PSBS16 制备的水泥（p=0.001）（46.6 与 36.7 MPa）。在 24 小时成熟后，不含氧化铝的 PSBS8 表现出 12.9 MPa 的弯曲强度，而商业对照值为 16.4 MPa。在成熟 1 天和 7 天后，不含氧化铝的 PSBS8 的强度高于含氧化铝的 PSBS16（p=0.001）。
本体外研究结果表明，不含氧化铝的硼硅酸盐生物活性玻璃比传统的 GIC 和含氧化铝的实验性硼硅酸盐玻璃更能增强牙髓细胞的活力、扩展和无细胞生物活性。
