Kim Dong-Ae, Lee Jung-Hwan, Jun Soo-Kyung, Kim Hae-Won, Eltohamy Mohamed, Lee Hae-Hyoung
Department of Biomaterials Science, School of Dentistry, Dankook University, Cheonan 330-714, South Korea.
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan 330-714, South Korea.
Dent Mater. 2017 Jul;33(7):805-817. doi: 10.1016/j.dental.2017.04.017. Epub 2017 May 20.
This study investigated the mechanical and in vitro biological properties (in immortalized human dental pulp stem cells (ihDPSCs)) of bioactive glass nanoparticle (BGN)-incorporated glass ionomer cement (GIC) with or without chitosan as a binder.
After the BGNs were synthesized and characterized, three experimental GICs and a control (conventional GIC) that differed in the additive incorporated into a commercial GIC liquid (Hy-bond, Shofu, Japan) were produced: BG5 (5wt% of BGNs), CL0.5 (0.5wt% of chitosan), and BG5+CL0.5 (5wt% of BGNs and 0.5wt% of chitosan). After the net setting time was determined, weight change and bioactivity were analyzed in simulated body fluid (SBF) at 37°C. Mechanical properties (compressive strength, diametral tensile strength, flexural strength and modulus) were measured according to the incubation time (up to 28 days) in SBF. Cytotoxicity (1day) and biomineralization (14 days), assessed by alizarin red staining, were investigated using an extract from GIC and ihDPSCs. Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey's post hoc test; p<0.05.
BGNs were sol-gel synthesized to be approximately 42nm in diameter with a spherical morphology and amorphous structure. After the bioactivity and suspension ability of the BGNs were confirmed, all the experimental GIC groups had setting times of less than 6min and approximately 1% weight loss after 28days of incubation. In addition, BGNs incorporated into GIC (BG5 and BG5+CL0.5) exhibited surface bioactivity. The mechanical properties were increased in the BGN-incorporated GICs compared to those in the control (p<0.05). Without cytotoxicity, the biomineralization capacity was ranked in the order BG5, BG5+CL0.5, control, and CL0.5 (p<0.05).
BGN-incorporated GIC showed enhanced mechanical properties such as compressive, diametral tensile and flexural strength as well as in vitro biomineralization properties in ihDPSCs without cytotoxicity. Therefore, the developed BGN-incorporated GIC is a promising restorative dental material, although further in vivo investigation is needed before clinical application.
本研究调查了添加或不添加壳聚糖作为粘结剂的生物活性玻璃纳米颗粒（BGN）增强型玻璃离子水门汀（GIC）的力学性能和体外生物学性能（在永生化人牙髓干细胞（ihDPSCs）中）。
合成并表征BGN后，制备了三种实验性GIC和一种对照（传统GIC），它们在添加到市售GIC液体（Hy-bond，日本松风）中的添加剂方面有所不同：BG5（5wt%的BGN）、CL0.5（0.5wt%的壳聚糖）和BG5+CL0.5（5wt%的BGN和0.5wt%的壳聚糖）。测定净凝固时间后，在37°C的模拟体液（SBF）中分析重量变化和生物活性。根据在SBF中的孵育时间（长达28天）测量力学性能（抗压强度、径向拉伸强度、弯曲强度和模量）。使用GIC和ihDPSCs的提取物研究细胞毒性（1天）和生物矿化（14天），通过茜素红染色进行评估。数据采用单因素方差分析（ANOVA），随后进行Tukey事后检验；p<0.05。
通过溶胶-凝胶法合成的BGN直径约为42nm，呈球形形态且为无定形结构。确认BGN的生物活性和悬浮能力后，所有实验性GIC组的凝固时间均小于6分钟，孵育28天后重量损失约1%。此外，添加到GIC中的BGN（BG5和BG5+CL0.5）表现出表面生物活性。与对照组相比，添加BGN的GIC的力学性能有所提高（p<0.05）。在无细胞毒性的情况下，生物矿化能力的排序为BG5、BG5+CL0.5、对照组和CL0.5（p<0.05）。
添加BGN的GIC在ihDPSCs中显示出增强的力学性能，如抗压强度、径向拉伸强度和弯曲强度，以及体外生物矿化性能，且无细胞毒性。因此，尽管在临床应用前需要进一步的体内研究，但所开发的添加BGN的GIC是一种有前景的牙科修复材料。
