Attik Nina, Basri Inès, Sohier Jérôme, Gauthier Rémy, Villat Cyril, Goutaudier Christelle
Laboratoire des Multimatériaux et Interfaces UMR CNRS 5615, Universite Claude Bernard Lyon 1, 6 rue Victor Grignard, 69622 Villeurbanne, France.
Faculté d'Odontologie de Lyon, 11 rue Guillaume Paradin, 69008 Lyon, France.
Materials (Basel). 2024 Nov 29;17(23):5862. doi: 10.3390/ma17235862.
Hydrogels are promising scaffolds for tissue regeneration, and borosilicate glass particles have demonstrated potential in enhancing the biological behaviour of dental pulp cells. However, the specific morphological characteristics of dental lesions and the diverse requirements of dental tissues require biocompatible, bioactive, and shapeable scaffolds. This study aimed to evaluate the in vitro biological behaviour of human gingival fibroblasts (HGFs) in contact with an experimental aluminum-free borosilicate glass-functionalized hydrogel. Two types of experimental borosilicate glass particles were utilized, with Biodentine particles serving as a reference material. The hydrogel, based on poly(L-lysine) dendrimers (DGL) with or without borosilicate particles, was analyzed using micro-computed tomography (µCT) and scanning electron microscopy (SEM) coupled with energy-dispersive X-ray spectroscopy (EDX). Cytocompatibility was assessed using Live/Dead™ staining, and cell colonization was evaluated via confocal imaging. Additionally, Alizarin red staining was performed to assess mineralization potential after 7 and 14 days. Results indicated that the incorporation of borosilicate particles did not alter hydrogel porosity, while EDX confirmed particle presence on the hydrogel surfaces. Furthermore, the borosilicate-functionalized hydrogels significantly enhanced cell proliferation, colonization, and the content of calcium deposits. These findings highlight the potential of these hydrogels for future clinical applications in dental tissue regeneration, pending further development.
水凝胶是用于组织再生的有前景的支架材料，硼硅酸盐玻璃颗粒已显示出增强牙髓细胞生物学行为的潜力。然而，牙齿病变的特定形态特征和牙齿组织的不同需求需要生物相容性好、具有生物活性且可塑形的支架。本研究旨在评估人牙龈成纤维细胞（HGFs）与一种实验性无铝硼硅酸盐玻璃功能化水凝胶接触后的体外生物学行为。使用了两种类型的实验性硼硅酸盐玻璃颗粒，以Biodentine颗粒作为参考材料。基于聚（L-赖氨酸）树枝状大分子（DGL）且含有或不含有硼硅酸盐颗粒的水凝胶，通过微计算机断层扫描（µCT）、扫描电子显微镜（SEM）以及能量色散X射线光谱（EDX）进行分析。使用活/死™染色评估细胞相容性，并通过共聚焦成像评估细胞定植情况。此外，进行茜素红染色以评估7天和14天后的矿化潜力。结果表明，硼硅酸盐颗粒的掺入并未改变水凝胶的孔隙率，而EDX证实了水凝胶表面存在颗粒。此外，硼硅酸盐功能化水凝胶显著增强了细胞增殖、定植以及钙沉积的含量。这些发现突出了这些水凝胶在牙齿组织再生未来临床应用中的潜力，有待进一步开发。
