State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China School of Stomatology, Sichuan Universitygrid.13291.38, Chengdu, China.
State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Conservation Dentistry and Endodontics, West China School of Stomatology, Sichuan Universitygrid.13291.38, Chengdu, China.
Microbiol Spectr. 2022 Aug 31;10(4):e0078222. doi: 10.1128/spectrum.00782-22. Epub 2022 Jul 13.
The cross-kingdom interactions between Candida albicans and Actinomyces viscosus play critical roles in root caries. However, the key pathway by which C. albicans regulates its interactions with A. viscosus is unclear. Here, we first employed 39 volunteers with root caries and 37 caries-free volunteers, and found that the abundances of C. albicans and A. viscosus were significantly increased in the individuals with root caries and showed a strong positive correlation. Their dual-species combination synergistically promoted biofilm formation and root caries in rats. The arginine biosynthesis pathway of C. albicans was significantly upregulated in dual-species biofilms and dental plaques from another 10 root caries volunteers compared with the 10 caries-free volunteers. The exogenous addition of arginine increased the cariogenicity of the dual-species biofilm. The C. albicans , a key gene from the arginine biosynthesis pathway, null mutant failed to promote dual-species biofilm formation and root caries in rats; however, the addition of arginine restored its synergistic actions with A. viscosus. Our results identified the critical roles of the C. albicans arginine biosynthesis pathway in its cross-kingdom interactions with A. viscosus for the first time and indicated that targeting this pathway was a practical way to treat root caries caused by multiple species. Root caries is a critical problem that threatens the oral health of the elderly population. Our results identified the essential roles of the C. albicans arginine biosynthesis pathway in its cross-kingdom interactions with A. viscosus in root caries for the first time and indicated that targeting this pathway was a practical way to treat root caries caused by multiple species.
白色念珠菌和黏性放线菌之间的跨界相互作用在根龋中起着关键作用。然而，白色念珠菌调节其与黏性放线菌相互作用的关键途径尚不清楚。在这里，我们首先招募了 39 名患有根龋的志愿者和 37 名无龋志愿者，发现白色念珠菌和黏性放线菌的丰度在根龋患者中显著增加，并表现出很强的正相关。它们的双种组合协同促进了大鼠生物膜的形成和根龋。与 10 名无龋志愿者相比，来自另外 10 名根龋志愿者的双种生物膜和牙菌斑中，白色念珠菌的精氨酸生物合成途径明显上调。外源性添加精氨酸增加了双种生物膜的致龋性。白色念珠菌精氨酸生物合成途径的关键基因缺失突变体未能促进大鼠双种生物膜的形成和根龋，而添加精氨酸恢复了其与黏性放线菌的协同作用。我们的研究结果首次确定了白色念珠菌精氨酸生物合成途径在其与黏性放线菌跨界相互作用中在根龋中的关键作用，并表明靶向该途径是治疗由多种物种引起的根龋的一种实用方法。根龋是威胁老年人口腔健康的一个关键问题。我们的研究结果首次确定了白色念珠菌精氨酸生物合成途径在其与黏性放线菌跨界相互作用中在根龋中的关键作用，并表明靶向该途径是治疗由多种物种引起的根龋的一种实用方法。
