State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Arch Oral Biol. 2019 Apr;100:106-112. doi: 10.1016/j.archoralbio.2019.02.008. Epub 2019 Feb 18.
How the interactions between Candida albicans and Actinomyces viscosus contributed to the root caries was not clear. This study aimed to investigate their cross-kingdom interactions on the biomass and the cariogenic virulence in dual-species biofilms.
Suspensions of C. albicans and A. viscosus were formed the mono and polymicrobial biofilms in vitro. Crystal violet assay, viable plate count, scanning electron microscopy and fluorescence in situ hybridization were used to analyze the biomass and biofilm structure. Glycolytic pH drop and the spectrophotometric method were used to evaluate the acid production and hydroxyapatite dissolution, respectively. The exopolysaccharide production was measured by the anthrone-sulfuric acid method, while the adhesion force was measured by atomic force microscopy.
The biomass and colony-forming units of mixed-species were significantly increased compared to that of the mono-species at 24 h, 48 h, 72 h. The structure of dual-species biofilm had more microcolonies and was much denser. The dual-species biofilms significantly decreased the pH value and damaged the hydroxyapatite compared with the mono-species biofilms at various time points, indicating the strong cariogenic virulence. Moreover, the dual-species biofilms significantly enhanced the exopolysaccharide production and adhesion force suggesting the increase of biofilm adhesion.
Cross-kingdom interactions of C. albicans and A. viscosus significantly elevated the biomass and cariogenic virulence of dual-species biofilm.
白色念珠菌和黏性放线菌之间的相互作用如何导致根龋尚不清楚。本研究旨在探讨它们在双物种生物膜中的跨界相互作用对生物量和致龋毒力的影响。
体外形成白色念珠菌和黏性放线菌的单种和混合生物膜悬液。结晶紫分析、活菌平板计数、扫描电子显微镜和荧光原位杂交用于分析生物量和生物膜结构。糖酵解 pH 值下降和分光光度法分别用于评估产酸和羟磷灰石溶解。采用蒽酮-硫酸法测量胞外多糖的产生，原子力显微镜测量粘附力。
与单种生物膜相比，混合生物膜在 24、48 和 72 小时时的生物量和菌落形成单位显著增加。双种生物膜的结构有更多的微菌落，而且更加密集。与单种生物膜相比，双种生物膜在各个时间点显著降低 pH 值并破坏羟磷灰石，表明其具有较强的致龋毒力。此外，双种生物膜显著增加了胞外多糖的产生和粘附力，表明生物膜的粘附力增加。
白色念珠菌和黏性放线菌的跨界相互作用显著提高了双种生物膜的生物量和致龋毒力。
