Takeuchi Y, Sakai T, Ikeya Y, Araki T, Ushio K, Yanagihara S
Sangyo Igaku. 1986 Nov;28(6):455-61. doi: 10.1539/joh1959.28.455.
A study was made on the effect of detection wavelength and separation mode of HPLC on determination of urinary hippuric acid (HA) and three isomers of methylhippuric acid (MHA). The interference of other constituents of urine in the determination was effectively decreased by detection at a short wavelength of 227.6 nm. Meta and para MHAs were separated by the addition of beta-cyclodextrine to the mobile phase. Four metabolites were successfully separated from other components of urine by the combination of ODS-silica packed-column and mobile phase (method F). The detection limits were found to be 50 and 5 mg/l for HA and MHAs, respectively. MHAs could not be detected in the non-exposed subjects. Average levels (+/- SD) of HA in non-exposed males and females were 272.2 (+/- 210.8) and 393.0 (+/- 269.8) mg/l, respectively. The urinary levels of HA in females were significantly higher than those in males.
研究了高效液相色谱法（HPLC）的检测波长和分离模式对尿中马尿酸（HA）及甲基马尿酸（MHA）三种异构体测定的影响。通过在227.6 nm的短波长处进行检测，有效降低了尿液中其他成分在测定中的干扰。通过在流动相中添加β-环糊精实现了间位和对位MHA的分离。采用ODS硅胶填充柱和流动相的组合（方法F）成功地从尿液的其他成分中分离出了四种代谢物。发现HA和MHA的检测限分别为50和5 mg/L。在未接触者中未检测到MHA。未接触的男性和女性中HA的平均水平（±标准差）分别为272.2（±210.8）和393.0（±269.8）mg/L。女性尿中HA水平显著高于男性。
