甲苯和二甲苯混合暴露后尿液中马尿酸和间甲基马尿酸的薄层色谱分析
Thin-layer chromatography of hippuric and m-methylhippuric acid in urine after mixed exposure to toluene and xylene.
作者信息
Bieniek G, Wilczok T
出版信息
Br J Ind Med. 1981 Aug;38(3):304-6. doi: 10.1136/oem.38.3.304.
Abstract
The separation of hippuric and m-methylhippuric acid as toluene and m-xylene metabolites present in urine of people exposed simultaneously to toluene and xylene is described. Chloroform was used for hippuric and m-methylhippuric acid extraction. Satisfactory separation of these metabolites was obtained on TLC plates covered with silica gels and developed in chloroform acetic acid-water (4:1:1);p-dimethylaminobenzaldehyde in acetic acid anhydride was applied to develop the colour. The sensitivity of the method was 6 micrograms hippuric acid per 1 ml urine and recovery was 100% (+/- 1).
摘要
描述了同时接触甲苯和二甲苯的人群尿液中作为甲苯和间二甲苯代谢物的马尿酸和间甲基马尿酸的分离方法。用氯仿提取马尿酸和间甲基马尿酸。在涂有硅胶的薄层色谱板上，以氯仿 - 乙酸 - 水（4:1:1）展开，可实现这些代谢物的良好分离；用乙酸酐中的对二甲氨基苯甲醛显色。该方法的灵敏度为每1 ml尿液中6微克马尿酸，回收率为100%（±1）。
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本文引用的文献
1
A simple method for simultaneous determination of benzoic and hippuric acids in biological fluids.
Clin Chim Acta. 1968 Feb;19(2):313-7. doi: 10.1016/0009-8981(68)90340-9.
2
A simple micromethod of determination of hippuric acid in the urine.
Biul Inst Med Morsk Gdansk. 1970;21(3):129-38.
3
Measurement of urinary hippuric and m-methylhippuric acids by gas chromatography.
Br J Ind Med. 1973 Apr;30(2):125-8. doi: 10.1136/oem.30.2.125.
4
Quantitative determination in urine of hippuric acid and m- or p-methylhippuric acid, metabolites of toluene and m- or p-xylene.
Br J Ind Med. 1969 Oct;26(4):330-4. doi: 10.1136/oem.26.4.330.
5
Simultaneous determination of toluene and xylene metabolites in urine by gas chromatography.
Br J Ind Med. 1977 Aug;34(3):229-33. doi: 10.1136/oem.34.3.229.
6
Urinary hippuric acid concentration after occupational exposure to toluene.
Br J Ind Med. 1978 Nov;35(4):330-4. doi: 10.1136/oem.35.4.330.
7
Absorption of m-xylene vapours through the respiratory tract and excretion of m-methylhippuric acid in urine.
Br J Ind Med. 1978 Feb;35(1):50-5.
8
Quantitative determination of hippuric and m-methylhippuric acids in urine by high-speed liquid chromatography.
Br J Ind Med. 1977 Nov;34(4):310-3. doi: 10.1136/oem.34.4.310.
9
Measurement by gas chromatography of urinary hippuric acid and methylhippuric acid as indices of toluene and xylene exposure.
Br J Ind Med. 1977 Nov;34(4):305-9. doi: 10.1136/oem.34.4.305.
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