da Costa Rosiane Andrade, Andrade Isadora Emanoela Pereira Costa, Pinto Otávio Henrique Bezerra, de Souza Beatriz Blenda Pinheiro, Fulgêncio Débora Luíza Albano, Mendonça Marise Leite, Kurokawa Adriane Silva, Ortega Daniel Barros, Carvalho Lucas Silva, Krüger Ricardo Henrique, Ramada Marcelo Henrique Soller, Barreto Cristine Chaves
Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Brasilia, SGAN 916, Brasília, DF, 70790-160, Brazil.
Laboratory of Enzymology, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, 70910-900, Brazil.
Amino Acids. 2022 Nov;54(11):1477-1489. doi: 10.1007/s00726-022-03187-9. Epub 2022 Jul 21.
Bacteria from the genus Paenibacillus make a variety of antimicrobial compounds, including lipopeptides produced by a non-ribosomal synthesis mechanism (NRPS). In the present study, we show the genomic and phenotypical characterization of Paenibacillus elgii AC13 which makes three groups of small molecules: the antimicrobial pelgipeptins and two other families of peptides that have not been described in P. elgii. A family of lipopeptides with [M + H] 1664, 1678, 1702, and 1717 m/z was purified from the culture cell fraction. Partial characterization revealed that they are similar to tridecaptin from P. terrae. However, they present amino acid chain modifications in positions 3, 7, and 10. These new variants were named tridecaptin G1, G2, G3, and G4. Furthermore, a gene cluster was identified in P. elgii AC13 genome, revealing high similarity to the tridecaptin-NRPS gene cluster from P. terrae. Tridecaptin G1 and G2 showed in vitro antimicrobial activity against Escherichia coli, Klebsiella pneumonia (including a multidrug-resistant strain), Staphylococcus aureus, and Candida albicans. Tri G3 did not show antimicrobial activity against S. aureus and C. albicans at all tested concentrations. An intriguing feature of this family of lipopeptides is that it was only observed in the cell fraction of the P. elgii AC13 culture, which could be a result of the amino acid sequence modifications presented in these variants.
类芽孢杆菌属的细菌能产生多种抗菌化合物，包括通过非核糖体合成机制（NRPS）产生的脂肽。在本研究中，我们展示了类芽孢杆菌AC13的基因组和表型特征，该菌株能产生三类小分子：抗菌肽pelgipeptins以及两类在类芽孢杆菌中尚未被描述过的肽。从培养细胞组分中纯化出了一组质荷比为[M + H] 1664、1678、1702和1717 m/z的脂肽。部分特征分析表明，它们与来自地衣芽孢杆菌的十三肽菌素相似。然而，它们在第3、7和10位呈现出氨基酸链修饰。这些新变体被命名为十三肽菌素G1、G2、G3和G4。此外，在类芽孢杆菌AC13基因组中鉴定出一个基因簇，与来自地衣芽孢杆菌的十三肽菌素-NRPS基因簇具有高度相似性。十三肽菌素G1和G2在体外对大肠杆菌、肺炎克雷伯菌（包括一株多重耐药菌株）、金黄色葡萄球菌和白色念珠菌具有抗菌活性。在所有测试浓度下，十三肽菌素G3对金黄色葡萄球菌和白色念珠菌均未表现出抗菌活性。这一族脂肽的一个有趣特征是，仅在类芽孢杆菌AC13培养物的细胞组分中观察到，这可能是这些变体中氨基酸序列修饰的结果。
