Mianyang Central Hospital, School of Medicine, University of Electronic Science and Technology of China, 12 Changjia Alley, Fucheng District, Mianyang, 621000, Sichuan, China.
J Interv Card Electrophysiol. 2022 Dec;65(3):663-673. doi: 10.1007/s10840-022-01305-x. Epub 2022 Jul 21.
In this study, we aimed to investigate the role of miR-26a and miR-26b in the management of AF.
Real-time PCR was carried out to determine plasma microRNA expression in AF patients pre- and post-radiofrequency ablation. The correlation between the expression of SELP and miR-26a/miR-26b was also studied using luciferase assays to establish a miR-26a/miR-26b/SELP signaling pathway.
The relative expression of SELP reached its peak in pre-ablation AF ( +) patients, while ablation treatment reduced the expression of SELP in AF ( +) patients. Similarly, AF pigs showed dysregulation of miR-26a/b and SELP, thus verifying the involvement of miR-26a/b and SELP in AF. Meanwhile, the regulatory association between SELP and miR-26a/b was also investigated, and the results showed that the presence of pre-miR-26a/b increased the levels of miR-26a/b and inhibited the mRNA/protein expression of SELP. Finally, using bioinformatic tools and luciferase assays, SELP mRNA was confirmed as the target of miR-26a/b, which affected the effect of AF ablation treatment.
RFA helped to restore circulating levels of miR-26, which were reduced in atrial fibrillation. Meanwhile, miR-26 is a potential cause for the elevated plasma levels of pro-thrombogenic SELP in that disease.
本研究旨在探讨 miR-26a 和 miR-26b 在房颤管理中的作用。
采用实时 PCR 法检测房颤患者射频消融术前后血浆 microRNA 表达，通过荧光素酶实验研究 SELP 与 miR-26a/miR-26b 的表达相关性，建立 miR-26a/miR-26b/SELP 信号通路。
SELP 的相对表达在消融前房颤（+）患者中达到峰值，而消融治疗降低了房颤（+）患者 SELP 的表达。同样，房颤猪也表现出 miR-26a/b 和 SELP 的失调，从而验证了 miR-26a/b 和 SELP 参与房颤。同时，还研究了 SELP 和 miR-26a/b 之间的调控关系，结果表明前 miR-26a/b 的存在增加了 miR-26a/b 的水平，并抑制了 SELP 的 mRNA/蛋白表达。最后，通过生物信息学工具和荧光素酶实验证实 SELP mRNA 是 miR-26a/b 的靶标，影响房颤消融治疗的效果。
RFA 有助于恢复房颤患者降低的循环 miR-26 水平。同时，miR-26 是导致该疾病中促血栓形成 SELP 血浆水平升高的潜在原因。
