基因组杂交捕获检测法：在马神经莱姆病诊断中的应用。
Genomic hybrid capture assay to detect : an application to diagnose neuroborreliosis in horses.
机构信息
College of Veterinary Medicine, Cornell University, Ithaca, NY, USA.
Institute of Genome Sciences, University of Maryland School of Medicine, Baltimore, MD, USA.
出版信息
J Vet Diagn Invest. 2022 Sep;34(5):909-912. doi: 10.1177/10406387221112617. Epub 2022 Jul 21.
Abstract
Antemortem diagnosis of neuroborreliosis in horses has been hindered by both the low sensitivity of PCR testing for in CSF and the low specificity of serum:CSF ELISA ratios used to determine intrathecal antibody production against the bacterium. PCR testing of the CSF of an adult horse with acute neurologic disease for the flagellin gene was negative. However, we enriched DNA through nucleic acid hybrid capture, followed by next-generation sequencing, and identified in the CSF of the horse, confirming a diagnosis of neuroborreliosis.
摘要
马的神经莱姆病的生前诊断受到 CSF 中 PCR 检测针对[病原体]的低灵敏度和用于确定针对细菌的鞘内抗体产生的血清：CSF ELISA 比值的低特异性的阻碍。对急性神经疾病的成年马的 CSF 进行的针对[病原体]鞭毛蛋白基因的 PCR 检测结果为阴性。然而，我们通过核酸杂交捕获对[病原体]DNA 进行了富集，然后进行了下一代测序，并在马的 CSF 中鉴定出了[病原体]，从而确诊了神经莱姆病。
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