Alizadeh Gita, Aryaeipour Mojgan, Mohebali Mehdi, Mowlavi Gholam Reza, Raissi Vahid, Rokni Mohammad Bagher
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Public Health. 2022 Mar;51(3):686-694. doi: 10.18502/ijph.v51i3.8947.
We aimed to compare semi-nested PCR with indirect ELISA to diagnose human fasciolosis.
Overall, 70 serum samples were collected from different areas in Iran suspected for fascioliasis. Individuals were classified based on diagnostic of fascioliasis and habitat in endemic areas. Finally, all serum samples were tested by indirect ELISA (using secretory excretory antigen) and semi-nested PCR (using ITS1 gene). The study was conducted in the School of Publish Health, Tehran University of Medical Sciences, Iran in 2021.
Significant differences were found between agreement and similarity of patients' results of indirect ELISA and semi-nested PCR 94.46% and 98.4% respectively (Cohen's kappa ≥0.6; -value≤0.05). No cross-reactions were observed with other parasitic diseases (toxocariasis, hydatidosis, strongyloidiasis, toxoplasmosis, cutaneous leishmaniasis, taeniasis and trichinosis). 69.84% of samples were positive by both techniques. In addition, the percentage of agreement and similarity between the results of the two techniques based on habitat in endemic areas was 88.9-100% and 97.7-100%, respectively (Cohen's kappa ≥0.6; -value≤0.05).
Semi-nested PCR could be a suitable method for following up on patients' treatment and a confirmatory method for ELISA as for diagnosis of human fascioliasis.
我们旨在比较半巢式PCR和间接ELISA用于诊断人体肝片吸虫病的效果。
总共从伊朗不同地区收集了70份疑似肝片吸虫病的血清样本。根据肝片吸虫病的诊断情况和流行地区的栖息地对个体进行分类。最后，所有血清样本均通过间接ELISA（使用分泌排泄抗原）和半巢式PCR（使用ITS1基因）进行检测。该研究于2021年在伊朗德黑兰医科大学公共卫生学院进行。
间接ELISA和半巢式PCR患者结果的一致性和相似性之间存在显著差异，分别为94.46%和98.4%（科恩kappa系数≥0.6；P值≤0.05）。未观察到与其他寄生虫病（弓蛔虫病、包虫病、粪类圆线虫病、弓形虫病、皮肤利什曼病、绦虫病和旋毛虫病）的交叉反应。两种技术检测均呈阳性的样本占69.84%。此外，基于流行地区栖息地的两种技术结果之间的一致性和相似性百分比分别为88.9 - 100%和97.7 - 100%（科恩kappa系数≥0.6；P值≤0.05）。
半巢式PCR可作为患者治疗随访的合适方法，以及ELISA诊断人体肝片吸虫病的确认方法。
