Aryaeipour Mojgan, Fard Ramin Mazaheri Nezhad, Rad Mohammad Bagher Molai, Pirestani Majid, Rouhani Soheila, Daryani Ahmad, Asadi Tina, Sarvi Shahabeddin, Rokni Mohammad Bagher
Student of Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2023 Apr-Jun;18(2):182-192. doi: 10.18502/ijpa.v18i2.13184.
Identification of the larval stages of spp. in freshwater snails is an essential guide to continue monitoring the possibility of their transmission and the potential of echinostomiasis in areas where trematodes are the primary agent of parasitic diseases. The aim of this study was investigate using morphological and molecular techniques.
The study was conducted in Gilan and Mazandaran Provinces, northern Iran, from April 2019 to October 2021. Overall, 5300 freshwater snails were randomly collected and were identified using external shell morphology. Meanwhile, snails infected with trematodes were studied via shedding and dissecting methods. Larvae stages of were identified and the genomic DNA of the samples was extracted. The PCR amplification of the ITSI gene was carried out for 17 isolates and products were sequenced. Seven sequences were deposited in GenBank.
Totally, 3.5% of snails containing three species ( sp., sp. and sp.) were infected with two types of cercaria with 37 and sp. with 45 spines in the collar. Moreover, 35% of the snails were infected with spp. metacercaria. Phylogenetic analysis illustrated that isolates were included in two ITSI haplogroups.
Results showed the potential hazard of a zoonotic parasite as in northern Iran. The potential of disease environmental relationship investigation and resource control optimization is necessary for effective disease prevention and health management.
鉴定淡水螺中 spp. 的幼虫阶段是持续监测其传播可能性以及在吸虫为主要寄生虫病病原体的地区开展棘口吸虫病防治工作的重要指南。本研究旨在利用形态学和分子技术进行调查。
该研究于2019年4月至2021年10月在伊朗北部的吉兰省和马赞德兰省进行。总共随机收集了5300只淡水螺，并通过外壳形态进行鉴定。同时，采用逸出法和解剖法对感染吸虫的螺进行研究。鉴定 spp. 的幼虫阶段，并提取样本的基因组DNA。对17个分离株进行 ITSI 基因的PCR扩增，并对产物进行测序。七个序列存入GenBank。
总共3.5% 的螺感染了两种尾蚴，其中包含三种螺（ sp.、 sp. 和 sp.），领口处分别有37个和45个棘。此外，35% 的螺感染了 spp. 囊蚴。系统发育分析表明，分离株包含在两个 ITSI 单倍群中。
结果显示了伊朗北部存在人畜共患寄生虫如 的潜在危害。为有效预防疾病和进行健康管理，有必要对疾病与环境的关系进行调查并优化资源控制。
