Talukdar Partha, Travis Anthony J, Hossain Mahmud, Islam Md Rafiqul, Norton Gareth J, Price Adam H
School of Biological Sciences University of Aberdeen Aberdeen UK.
Department of Soil Science Bangladesh Agricultural University Mymensingh Bangladesh.
Food Energy Secur. 2022 Feb;11(1):e329. doi: 10.1002/fes3.329. Epub 2021 Sep 30.
Iron (Fe) deficiency is one of the common causes of anaemia in humans. Improving grain Fe in rice, therefore, could have a positive impact for humans worldwide, especially for those people who consume rice as a staple food. In this study, 225-269 accessions of the Bengal and Assam Aus Panel (BAAP) were investigated for their accumulation of grain Fe in two consecutive years in a field experiment under alternative wetting and drying (AWD) and continuous flooded (CF) irrigation. AWD reduced straw Fe by 40% and grain Fe by 5.5-13%. Genotype differences accounted for 35% of the variation in grain Fe, while genotype by irrigation interaction accounted for 12% of the variation in straw and grain Fe in year 1, with no significant interactions detected in year 2. Twelve rice accessions were identified as having high grain Fe for both years regardless of irrigation treatment, half of which were from BAAP subgroup 3 which prominently comes from Bangladesh. On average, subgroup 3 had higher grain Fe than the other four subgroups of . Genome-wide association mapping identified 6 genomic loci controlling natural variation of grain Fe concentration in plants grown under AWD. For one QTL, nicotianamine synthase is proposed as candidate for controlling natural variation of grain Fe in rice. The BAAP contains three haplotypes of where one haplotype (detected in 31% of the individuals) increased grain Fe up to 11%. Haplotype analysis of this gene in rice suggests that the ability to detect the QTL is enhanced in the BAAP because the high Fe allele is balanced in , unlike and subgroups.
缺铁是人类贫血的常见原因之一。因此，提高水稻籽粒中的铁含量可能会对全球人类产生积极影响，特别是对于那些以大米为主食的人群。在本研究中，在交替湿润和干燥（AWD）及持续淹水（CF）灌溉的田间试验中，连续两年对225 - 269份孟加拉和阿萨姆奥氏稻种资源库（BAAP）材料的籽粒铁积累情况进行了调查。AWD使秸秆铁含量降低了40%，籽粒铁含量降低了5.5% - 13%。基因型差异占籽粒铁含量变异的35%，而基因型与灌溉的互作在第1年占秸秆和籽粒铁含量变异的12%，第2年未检测到显著互作。无论灌溉处理如何，有12份水稻材料在两年中均被鉴定为具有高铁含量籽粒，其中一半来自主要源自孟加拉国的BAAP亚群3。平均而言，亚群3的籽粒铁含量高于其他四个亚群。全基因组关联图谱鉴定出6个控制AWD条件下生长植物籽粒铁浓度自然变异的基因组位点。对于一个QTL，推测烟酰胺合酶是控制水稻籽粒铁自然变异的候选基因。BAAP包含三个该基因的单倍型，其中一个单倍型（在31%的个体中检测到）使籽粒铁含量提高了11%。对水稻中该基因的单倍型分析表明，在BAAP中检测该QTL的能力增强，因为与其他亚群不同，高铁等位基因在该亚群中是平衡的。
