School of Biomedical Sciences, The University of Western Australia, Crawley, 6009, Australia.
School of Biomedical Sciences, The University of Western Australia, Crawley, 6009, Australia; Cooperative Research Centre for Honey Bee Products Limited (CRC HBP), 128 Yanchep Beach Road, Yanchep, 6035, Australia.
Complement Ther Clin Pract. 2022 Nov;49:101640. doi: 10.1016/j.ctcp.2022.101640. Epub 2022 Jul 19.
Impetigo is a contagious skin disease caused by Staphylococcus aureus and Streptococcus pyogenes. Without treatment, impetigo may be recurrent, develop into severe disease, or have serious, life-threatening sequelae. Standard treatment consists of topical or systemic antibiotic therapy (depending on severity), however, due to antibiotic resistance some therapies are increasingly ineffective. In this study we evaluated the potential for honey as an alternative treatment for impetigo. A broth microdilution assay in 96-well microtitre trays was used to determine the minimum inhibitory concentrations (MICs) of six monofloral honeys (jarrah, marri, red bell, banksia, wandoo, and manuka), a multifloral honey and artificial honey against S. aureus (n = 10), S. pyogenes (n = 10), and coagulase-negative staphylococci (CoNS) (n = 10). The optical density (OD) of all microtitre tray wells was also determined before and after assay incubation to analyse whether sub-MIC growth inhibition occurred. Jarrah, marri, red bell, banksia, and manuka honeys were highly effective at inhibiting S. aureus and CoNS, with MIC values ranging from 4 to 8% w/v honey. S. pyogenes was also inhibited by these same honeys, albeit at higher concentrations (8-29% w/v). Wandoo and multifloral honeys had the least antibacterial activity with MICs of >30% (w/v) for all isolates. However, OD data indicated that sub-MIC concentrations of honey were still partially restricting bacterial growth. Our pre-clinical data indicate that honey may be a potential therapeutic agent for the routine treatment of mild impetigo, and we suggest that clinical trials would be appropriate to further investigate this.
脓疱疮是一种由金黄色葡萄球菌和化脓性链球菌引起的传染性皮肤病。未经治疗，脓疱疮可能会反复发作、发展为严重疾病，或产生严重的、危及生命的后遗症。标准治疗包括局部或全身抗生素治疗（取决于严重程度），但是，由于抗生素耐药性，一些治疗方法的效果越来越差。在这项研究中，我们评估了蜂蜜作为脓疱疮替代治疗方法的潜力。采用 96 孔微量滴定板肉汤微量稀释法测定六种单花蜜（尤加利、桃金娘、红钟花、班克西亚、沃杜和麦卢卡）、一种百花蜜和人工蜜对金黄色葡萄球菌（n=10）、化脓性链球菌（n=10）和凝固酶阴性葡萄球菌（CoNS）（n=10）的最小抑菌浓度（MIC）。在测定孵育前后，所有微量滴定板孔的光密度（OD）也进行了测定，以分析是否发生亚 MIC 生长抑制。尤加利、桃金娘、红钟花、班克西亚和麦卢卡花蜜对金黄色葡萄球菌和 CoNS 的抑制作用非常有效，其 MIC 值范围为 4%至 8%w/v 蜂蜜。这些花蜜也抑制化脓性链球菌，尽管浓度较高（8%至 29%w/v）。沃杜和百花蜜的抗菌活性最低，对所有分离株的 MIC 值均大于 30%（w/v）。然而，OD 数据表明，蜂蜜的亚 MIC 浓度仍部分限制了细菌的生长。我们的临床前数据表明，蜂蜜可能是治疗轻度脓疱疮的一种潜在治疗剂，我们建议进行临床试验以进一步对此进行研究。
