Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium; Center for Microbiology, VIB, 3000 Leuven, Belgium.
Laboratory of Molecular Bacteriology, Department of Microbiology and Immunology, Rega Institute, KU Leuven, Leuven, Belgium; Center for Microbiology, VIB, 3000 Leuven, Belgium.
Cell. 2022 Jul 21;185(15):2725-2738. doi: 10.1016/j.cell.2022.06.040.
Microbial culturing and meta-omic profiling technologies have significantly advanced our understanding of the taxonomic and functional variation of the human microbiome and its impact on host processes. The next increase in resolution will come by understanding the role of low-abundant and less-prevalent bacteria and the study of individual cell behaviors that underlie the complexity of microbial ecosystems. To this aim, single-cell techniques are being rapidly developed to isolate, culture, and characterize the genomes and transcriptomes of individual microbes in complex communities. Here, we discuss how these single-cell technologies are providing unique insights into the biology and behavior of human microbiomes.
微生物培养和元组学分析技术极大地促进了我们对人类微生物组的分类和功能变化及其对宿主过程影响的理解。下一个分辨率的提高将来自于理解低丰度和较少流行的细菌的作用，以及研究构成微生物生态系统复杂性的单个细胞行为。为此，单细胞技术正在迅速发展，以分离、培养和表征复杂群落中单个微生物的基因组和转录组。在这里，我们讨论了这些单细胞技术如何为人类微生物组的生物学和行为提供独特的见解。
