School of Pharmacy, Anhui Medical University, Hefei, 230032, PR China.
School of Pharmacy, Anhui Medical University, Hefei, 230032, PR China.
Anal Chim Acta. 2022 Aug 8;1220:340070. doi: 10.1016/j.aca.2022.340070. Epub 2022 Jun 11.
In this work, novel luminol functionalized nanoparticles (SnO@Luminol NPs) with mesoporous spherical structure were synthesized by a simple and green method, which exhibited good chemiluminescence (CL) intensity without any additional catalysts. In addition, the CL intensities generated by SnO@Luminol/HO system were linearly related to the logarithm values of HO concentrations, and CL mechanism of SnO@Luminol/HO system was investigated. On this basis, a sensitive CL method was proposed to determine glucose and uric acid. The fabricated CL method displayed wide linear ranges (glucose, 1.0 × 10 - 1.0 × 10 M; uric acid, 1.0 × 10 - 1.0 × 10 M) and low detection limits (glucose, 5.4 nM; uric acid, 6.8 nM). The proposed CL method achieved sensitive and specific glucose and uric acid detection in human serum specimens, and the measured results were consistent with the clinical diagnosis. Furthermore, as long as the product of reaction between enzyme and substrate is HO, this CL method can also be extended to other biological detection fields.
在这项工作中，通过一种简单、绿色的方法合成了具有介孔球形结构的新型鲁米诺功能化纳米粒子（SnO@Luminol NPs），无需任何额外的催化剂即可表现出良好的化学发光（CL）强度。此外，SnO@Luminol/HO 体系产生的 CL 强度与 HO 浓度的对数呈线性关系，并对 SnO@Luminol/HO 体系的 CL 机制进行了研究。在此基础上，提出了一种灵敏的 CL 方法来测定葡萄糖和尿酸。所构建的 CL 方法具有较宽的线性范围（葡萄糖，1.0×10 - 1.0×10 M；尿酸，1.0×10 - 1.0×10 M）和较低的检测限（葡萄糖，5.4 nM；尿酸，6.8 nM）。该 CL 方法在人血清标本中实现了对葡萄糖和尿酸的灵敏和特异性检测，且测量结果与临床诊断一致。此外，只要酶和底物反应的产物是 HO，该 CL 方法也可以扩展到其他生物检测领域。
