State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Analyst. 2020 Apr 7;145(7):2709-2715. doi: 10.1039/c9an02600b. Epub 2020 Feb 20.
Herein, we report luminol-silicotungstic acid (STA) chemiluminescence (CL) for the first time. The luminol-STA system resulted in remarkable CL enhancement (65 times) compared with the known classical luminol-HO system because of the generation of the strong oxidizing agent tungsten trioxide from STA. Based on the quenching effect of uric acid, the new CL system is applied for the sensitive and selective assay of uric acid in its pure state (LOD 0.75 nM) and in real human urine with excellent recoveries in the range of 99.6-102.3%. Furthermore, this system permits the efficient detection of STA (LOD, 0.24 μM).
本文首次报道了鲁米诺-硅钨酸（STA）化学发光（CL）。由于 STA 生成了强氧化剂三氧化钨，与已知的经典鲁米诺-HO 体系相比，鲁米诺-STA 体系产生了显著的 CL 增强（65 倍）。基于尿酸的猝灭效应，新的 CL 体系可用于尿酸的灵敏和选择性测定，在纯态（LOD0.75 nM）和真实人尿中的回收率均在 99.6-102.3%范围内。此外，该体系还可以有效地检测 STA（LOD，0.24 μM）。
