Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Menglun, Mengla, Yunnan 666303, China; University of Chinese Academy of Sciences, Beijing 100049, China.
School of Pharmacy, State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, Guizhou 550004, China.
Carbohydr Polym. 2022 Oct 15;294:119777. doi: 10.1016/j.carbpol.2022.119777. Epub 2022 Jun 25.
Aspidopterys obcordata vine is a Chinese Dai ethnic herb used to treat urolithiasis. However, the material basis and underlying mechanisms remain undefined. In this study, a 2.3 kD inulin-like A. obcordata fructan (AOFOS) was isolated by size exclusion column chromatography and characterized by ultrahigh-performance liquid chromatography-ion trap-time of flight mass spectrometry (UPLC-IT-TOF-MS), nuclear magnetic resonance (NMR) spectroscopy, gas chromatography mass spectrometry (GC-MS) and high-performance gel permeation chromatography (HGPC). In addition, AOFOS showed unique anti-urolithiasis activity in Drosophila kidney stone models. Mechanism study indicated that AOFOS reduced the size of calcium oxalate crystals by inhibiting the formation of large size crystals and the generation rate of calcium oxalate crystals as well as the crystal form conversion from calcium oxalate monohydrate (COM) to calcium oxalate dihydrate (COD).
扁枝崖角藤藤茎是中国傣族民间用于治疗尿路结石的草药。然而，其物质基础和作用机制尚不清楚。本研究采用排阻色谱柱层析法分离得到 2.3kDa 的菊苣型崖角藤果聚糖（AOFOS），并通过超高效液相色谱-离子阱飞行时间质谱联用（UPLC-IT-TOF-MS）、核磁共振波谱（NMR）、气相色谱-质谱联用（GC-MS）和高效凝胶渗透色谱（HGPC）对其进行了表征。此外，AOFOS 在果蝇肾结石模型中表现出独特的抗结石活性。机制研究表明，AOFOS 通过抑制大尺寸晶体的形成、草酸钙晶体的生成速率以及草酸钙一水合物（COM）向草酸钙二水合物（COD）的晶体形态转化，从而减小草酸钙晶体的尺寸。
