Institute for Solid State Physics, University of Tokyo, Kashiwa, Chiba 277-8581, Japan.
J Chem Phys. 2022 Jul 21;157(3):034901. doi: 10.1063/5.0098249.
Curvature-inducing proteins containing a bin/amphiphysin/Rvs domain often have intrinsically disordered domains. Recent experiments have shown that these disordered chains enhance curvature sensing and generation. Here, we report on the modification of protein-membrane interactions by disordered chains using meshless membrane simulations. The protein and bound membrane are modeled together as a chiral crescent protein rod with two excluded-volume chains. As the chain length increases, the repulsion between them reduces the cluster size of the proteins. It induces spindle-shaped vesicles and a transition between arc-shaped and circular protein assemblies in a disk-shaped vesicle. For flat membranes, an intermediate chain length induces many tubules owing to the repulsion between the protein assemblies, whereas longer chains promote perpendicular elongation of tubules. Moreover, protein rods with zero rod curvature and sufficiently long chains stabilize the spherical buds. For proteins with a negative rod curvature, an intermediate chain length induces a rugged membrane with branched protein assemblies, whereas longer chains induce the formation of tubules with periodic concave-ring structures.
含有双域/ amphiphysin/Rvs 结构域的诱导曲率蛋白通常含有无规则结构域。最近的实验表明，这些无规则链增强了曲率感应和产生。在这里，我们使用无网格膜模拟报告了无序链对蛋白质-膜相互作用的修饰。将蛋白质和结合的膜一起建模为具有两个排斥体积链的手性新月形蛋白棒。随着链长的增加，它们之间的排斥力降低了蛋白质的簇大小。它诱导出纺锤形囊泡，并在盘状囊泡中诱导出弧形和圆形蛋白质组装之间的转变。对于扁平膜，由于蛋白质组装之间的排斥，中间链长会诱导出许多小管，而较长的链则会促进管状物的垂直伸长。此外，具有零棒曲率和足够长链的蛋白质棒稳定了球形芽。对于具有负棒曲率的蛋白质，中间链长会诱导具有分支蛋白质组装的粗糙膜，而较长的链会诱导具有周期性凹环结构的管状物的形成。
